中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (3): 168-169.

• 论著 • 上一篇    下一篇

喜树碱抗血管生成作用的研究

刘晓明1, 安利佳2, 王红梅1, 包永明2, 段延龙2, 林熙然1   

  1. 1. 大连医科大学附属第一医院皮肤科 116011;
    2. 大连理工大学生物工程系
  • 收稿日期:2004-01-12 出版日期:2005-03-15 发布日期:2005-03-15
  • 基金资助:
    辽宁省自然科学基金资助项目(2040736)

Potential Antiangiogenic Effect of Camptothecin in vitro

LIU Xiao-ming1, AN Li-jia2, WANG Hong-mei1, BAO Yong-ming2, DUAN Yan-long2, LIN Xi-ran1   

  1. Department of Dermatology, The First Affliated Hospital of Dalian Medical University, Institute of Bioengineering, Dalian University of Technology. Dalian 116011, China
  • Received:2004-01-12 Online:2005-03-15 Published:2005-03-15

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摘要： 目的 探讨喜树碱在体外对人脐静脉血管内皮细胞ECV304增殖抑制和凋亡诱导作用。方法 将不同浓度的喜树碱作用于ECV304细胞株,用MTT法、光镜、电镜、DNA梯状带检测药物对细胞的增殖抑制和凋亡诱导作用。结果 喜树碱能抑制ECV304细胞的增殖。24h,36h,48h最大抑制率分别为67.9%,96.7%,97.2%。形态学观察细胞生长受抑制,可见凋亡小体。凝胶电泳显示典型的DNA梯度带。结论 喜树碱体外能抑制人脐静脉血管内皮细胞增殖并诱导其凋亡,证明喜树碱可能具有抗血管生成作用。

关键词: 喜树碱, 细胞凋亡, 内皮,血管

Abstract: Objective To investigate the effect of camptothecin (CPT) on the inhibition of proliferation and induction of apoptosis of human umbilical vein endothelial cell line,ECV304,in vitro.Methods ECV304s were incubated with different concentrations of CPT.The inhibitory effect on cellular proliferation was measured by MTT assay.Cellular apoptosis was evaluated by light microscopy,electron microscopy,flow cytometry,and agarose gel electrophoresis.Results CPT could inhibit the proliferation of ECV304.The maximal inhibition rates were 67.9%,96.7%,and 97.2% at 24 h,48 h and 72 h,respectively.Having been treated with CPT,ECV304 cells were found to have typical morphologic features of apoptosis.TheResultsof agarose gel electrophoresis showed characteristic DNA ladder after ECV304 were treated with CPT.Conclusions CPT shows inhibitory effect on the proliferation of ECV304 and can induce apoptosis of ECV304.CPT may have a potential antiangiogenic effect.

Key words: Camptothecin, Apoptosis, Endothelium,vascular