中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (2): 102-104.

• 论著 • 上一篇    下一篇

何首乌、女贞子等中药煎剂对体外培养的猪毛囊毛发生长的影响

张兴洪, 范卫新   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:2004-02-12 出版日期:2005-02-15 发布日期:2005-02-15
  • 基金资助:
    国家自然科学基金资助项目(30170860);江苏省中医药管理局基金资助项目(9975);江苏省科委基金资助项目(99039)

Effect of Some Traditional Chinese Herbs on Hair Growth of Pig Hair Follicle in vitro

ZHANG Xing-hong, FAN Wei-xin   

  1. Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2004-02-12 Online:2005-02-15 Published:2005-02-15

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摘要： 目的 探讨何首乌、女贞子等中药混合煎剂对体外培养的猪毛囊毛发生长的影响。方法 将离体培养的猪毛囊分为对照组(Williams E培养基)和中药组(Williams E培养基+中药煎剂),显微镜下观察各组毛囊毛发生长和毛球部形态变化,并用末端脱氧核苷酸转移酶介导dUTP缺口末端标记法(TUNEL)检测各组毛囊中的凋亡细胞。结果 培养第7天,对照组毛囊毛发生长长度低于中药组(P<0.001);对照组毛囊TUNEL阳性细胞数明显高于中药组(P<0.001);毛球形态观察,对照组毛囊出现退行性变化,而中药组毛囊仍维持生长期毛囊形态。结论 何首乌、女贞子等中药煎剂能在一定程度上抑制猪毛囊细胞内凋亡,延缓生长期毛囊进入退行期。

关键词: 中草药, 毛囊, 细胞凋亡

Abstract: Objective To investigate the effect of water soluble extracts of some traditional Chinese herbs on hair growth of pig hair follicle in vitro. Methods Pig hair follicles were cultured with Williams E medium (control group) or Williams E medium with water soluble extracts of Chinese herbs (experimental group). Hair growth and morphological changes in the hair follicle bulb were observed by microscope, and apoptosis of the pig hair follicle cells was detected by a TUNEL technique. Results On day 7 of culture, the hair growth in the control group was slower than that in the experimental group (P<0.001), and apoptosis in hair follicle in the control group was significantly more than that in the experimental group (P<0.001). The morphology study showed that catagen/telogen stages occurred in the hair follicles in the control group, while the hair follicles in the experimental group were still maintaining in anagen stages. Conclusion These results suggest that water soluble extracts of some traditional Chinese herbs can partially inhibit the apoptosis of the pig hair follicle cells and delay the development from anagen stages to catagen/telogen stages of hair follicles.

Key words: Drugs, Chinese herbal, Hair follicle, Apoptosis