中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (12): 725-728.

• 论著 • 上一篇    下一篇

二期梅毒患者外周血淋巴细胞凋亡及其Fas、Bcl-2表达的研究

曾文军, 樊翌明, 吴志华   

  1. 广东医学院附属医院皮肤科 湛江 524001
  • 收稿日期:2005-01-24 发布日期:2005-12-15

Peripheral Blood Lymphocytes Apoptosis and Expression of Fas and Bcl-2 in Patients with Secondary Syphilis

ZENG Wen-jun, FAN Yi-ming, WU Zhi-hua   

  1. Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
  • Received:2005-01-24 Published:2005-12-15

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摘要： 目的 检测二期梅毒患者外周血淋巴细胞(PBLC)凋亡及其Fas、Bcl-2的表达,探讨二期梅毒患者免疫功能异常与淋巴细胞凋亡的关系。方法 采用流式细胞仪检测33例二期梅毒患者和30例正常人PBLC细胞凋亡及其Fas、Bcl-2的表达。结果 梅毒患者PBLC及CD4+细胞Fas表达明显高于对照组(P<0.01),而Bcl-2表达明显低于对照组(P<0.01)。CD8+、CD19+细胞Fas、Bcl-2表达两组比较差异无统计学意义(P>0.05)。梅毒患者PBLC及CD4+细胞凋亡率显著高于对照组(P<0.01),CD8+及CD19+细胞凋亡率两组比较差异无统计学意义(P>0.05)。梅毒患者PBLC及CD4+细胞凋亡率分别与其Fas表达呈显著正相关(r=0.68,P<0.01;r=0.71,P<0.01),但与Bcl-2表达呈显著负相关(r=-0.82,P<0.01;r=-0.74,P<0.01)。结论 二期梅毒患者细胞免疫功能异常可能与淋巴细胞凋亡过度有关,而淋巴细胞凋亡过度与Fas、Bcl-2表达异常有关。

关键词: 梅毒, 淋巴细胞亚群, 细胞凋亡, 抗原,CD95, 原癌基因蛋白质c-bcl-2

Abstract: Objective To investigate the relationship between apoptosis of peripheral blood lymphocytes (PBLCs) and impaired immunity by assessing the apoptosis and expression of Fas and Bcl-2 in PBLCs of patients with secondary syphilis. Methods Flow cytometry (FCM) was used to detect PBLC apoptosis as well as Fas and Bcl-2 expression in 33 patients with secondary syphilis and 30 normal controls. Results The Fas expression on PBLCs and CD4+ cells was significantly higher in patients than that in controls (P<0.01), which was contrary to the Bcl-2 expression. No significant difference was found in either Fas or Bcl-2 expression on CD8+ or CD19+ cells between patients and controls (P>0.05). The apoptosis levels were significantly higher in PBLCs and CD4+ cells in patients than those in controls (P<0.01), and were not different in the CD8+ or CD 19+ cells between patients and controls (P>0.05). In patients group, the apoptosis levels of both PBLCs and CD4+ cells had a positive correlation with the Fas expression (r=0.68, P<0.01;r=0.71, P<0.01, respectively), but a negative correlation with the Bcl-2 expression (r=-0.82, P<0.01;r=-0.74, P<0.01, respectively). Conclusions Dysfunction of cellular immunity may be due to the increased PBLC apoptosis in patients with secondary syphilis, which is associated with the abnormal expression of Fas and Bcl-2 on PBLCs.

Key words: Syphilis, Lymphocyte subsets, Apoptosis, Antigens, CD95, Proto-oncogene proteins c-bcl-2