中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (12): 722-724.

• 论著 • 上一篇    下一篇

聚合酶链反应快速诊断浅部真菌病

李筱芳, 陈辉, 崔凡, 陈伟, 吕桂霞, 沈永年, 刘维达   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2005-02-02 发布日期:2005-12-15
  • 通讯作者: 刘维达,E-mail:liumyco@hotmail.com E-mail:liumyco@hotmail.com
  • 基金资助:
    江苏省卫生厅医学科技发展基金重大课题(H200203)

Direct Detection of Pathogenic Fungi in Superficial Fungal Infections Based on Polymerase Chain Reaction

LI Xiao-fang, CHEN Hui, CUI Fan, CHEN Wei, LÜ Gui-xia, SHEN Yong-nian, LIU Wei-da   

  1. Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2005-02-02 Published:2005-12-15

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摘要： 目的 评价聚合酶链反应(PCR)技术直接从临床标本中检测浅部真菌病病原真菌的方法。方法 采用蛋白酶K消化法和煮沸法处理临床标本,从中提取致病菌DNA,然后用真菌通用引物ITS1进行PCR扩增检测,并与直接镜检和培养法作对比。结果 共收集112例临床标本,PCR检测、直接镜检和培养的敏感性分别为80.7%、96.5%和70.2%,特异性分别为100%、89.1%和100%,阳性预测值分别为100%、90.2%和100%,阴性预测值分别为83.3%、96.1%和76.4%。PCR方法在24h内即可完成从标本处理至结果判读的全过程。结论 PCR检测具有较好的特异性和准确性,且比培养缩短了近2周的时间,为临床快速诊断和及时、合理地治疗浅部真菌病提供参考。

关键词: 皮肤真菌病, 聚合酶链反应, 诊断

Abstract: Objective To develop a rapid and reliable polymerase chain reaction (PCR) procedure to detect the pathogenic fungi in clinical specimens. Methods Skin, nail and hair samples were taken from patients suspected of being infected with superficial mycosis. Pathogens were detected by PCR based on the ITS1 primer, and the results were compared with those from microscopic examination and culture. Results One hundred and twelve patients were recruited in this study. For PCR, microscopic examination and culture the sensitivities were 80.7%, 96.5% and 70.2%, the specificities were 100%, 89.1% and 100%, the positive predictive values were 100%, 90.2% and 100%, and the negative predictive values were 83.3%, 96.1% and 76.4%, respectively. The PCR process could be completed within 24 h. Conclusions PCR assay has good specificity and accuracy, while fungal culture takes 2 weeks to get the results. PCR is helpful for making rapid clinical diagnosis, which leads to the appropriate treatment of superficial fungal infection.

Key words: Dennatomycoses, Polymerase chain reaction, Diagnosis