中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (11): 659-661.

• 论著 • 上一篇    下一篇

角蛋白17对银屑病患者T细胞增殖和分泌干扰素γ的影响

沈柱, 王刚, 李巍, 刘玉峰   

  1. 第四军医大学西京医院皮肤科 西安 710032
  • 收稿日期:2003-11-01 出版日期:2004-11-15 发布日期:2004-11-15
  • 基金资助:
    国家自然科学基金(30371650)

The Impacts of Keratin 17 on the Excretion of IFN-γ and the Proliferation of T Lymphocytes from Patients with Psoriasis

SHEN Zhu, WANG Gang, LI Wei, LIU Yu-feng   

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China
  • Received:2003-11-01 Online:2004-11-15 Published:2004-11-15

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摘要： 目的 检测人角蛋白17对银屑病患者T细胞增殖和细胞因子分泌的影响。方法 从银屑病皮损中提取总RNA,反转录成cDNA,通过PCR方法扩增人角蛋白17基因,利用GST融合表达载体pGEX-4T-1表达相应融合蛋白;梯度离心和绵羊红细胞花环沉淀法分离纯化T细胞;直接活细胞计数和MTT法检测T细胞增殖程度,ELISA检测培养上清液中干扰素γ浓度。结果 和正常人对照相比,角蛋白17可以显著促进银屑病患者T细胞的增殖,并且促进其分泌干扰素γ(P值均<0.001)。结论 角蛋白17对银屑病患者T细胞功能的促进作用提示其可能是引发银屑病的重要自身抗原之一。

关键词: 银屑病, 角蛋白, 干扰素Ⅱ型

Abstract: Objective To investigate the immunological effects of human keratin 17 (K17) on T lymphocytes from psoriatic patients.Methods Total RNA was extracted from the epidermis of psoriatic lesions,and mRNA was reversely transcribed to cDNA.The K17 gene was amplified by PCR and inserted into pGEX-4T-1 expression vector,and then K17 was expressed and purified.T cells were isolated and purified by gra-dient centrifugation and AET-SRBC (2-aminoethylosothiouronium bromide-treated sheep red blood cells) precipitation.MTT assay and direct cell counting method were used to detect the proliferation of T cells.The concentration of IFN-γ in culture supernatant was measured by ELISA.Results Compared with the control group,K17 significantly enhanced the proliferation of T cells from psoriatic patients and increased the expression level of IFN-γ(P<0.001 respectively).Conclusion K17 can enhance the function of T cells in psoriatic patients,and it may play a role as an autoantigen in the immunological pathogenesis of psoriasis.

Key words: Psoriasis, Keratin, Interferon typeⅡ