中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 293-295.

• 论著 • 上一篇    下一篇

肿瘤坏死因子α对角质形成细胞产生白介素8和增殖活性的影响

陈先进, 张开明, 尹国华, 李慧仙   

  1. 山西省太原市中心医院皮肤科 030009
  • 收稿日期:2001-07-05 出版日期:2002-08-15 发布日期:2002-08-15

Effects of TNF-α,sTNFRⅠon IL-8Production and Proliferation of Psoriatic Keratinocytes

CHEN Xianjin, ZHANG Kaiming, YIN Guohua, LI Huixian   

  1. Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, China
  • Received:2001-07-05 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 了解肿瘤坏死因子α(TNF-α)、肿瘤坏死因子受体Ⅰ(TNFRⅠ)和抗TNFRⅠ抗体对银屑病患者角质形成细胞(KC)产生白介素8(IL-8)和增殖活性的影响.方法 分离表皮细胞接种96孔板,分别为:①自然增殖孔;②TNF-α刺激孔;③sTNFRⅠ/TNFα培养孔;④sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔;⑤抗TNFRⅠ/TNF-α培养孔.ELISA法测上清液IL-8的含量;MTT法测增殖活性;收集细胞提取RNA进行反转录和扩增,产物电泳后转膜,进行DNA印迹以比较IL-8的表达在转录水平上的差异.结果 银屑病患者组所测3个指标均较正常对照组高(P<0.05);两组TNF-α刺激孔KC3个指标均显着高于自然增殖孔和sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔;自然增殖孔和sTNFRⅠ/抗TNFRⅠ/TNF-α培养孔之间比较差异无显着性.结论 ①银屑病患者KC处于活化状态,分泌高水平细胞因子且增殖活跃;②TNF-α可诱导体外培养KC表达IL-8mRNA和蛋白,促进KC增殖,其作用可能主要是通过和细胞表面TNFRⅠ结合来实现;③sTNFRⅠ可以部分阻断TNF-α的生物学作用.

关键词: 银屑病, 肿瘤坏死因子, 白细胞介素8, 角蛋白细胞

Abstract: Objective To study the effects of TNF-αand sTNFRⅠon IL-8 production and proliferation of psoriatic keratinocytes.Methods In vitro cultured keratinocytes were treated with TNF-α,sTNFRⅠand anti-TNFRⅠ.IL-8 levels in supernatants were me asured by enzyme-linked immunosobent assay.Expression of IL-8 mRNA in keratinocytes was observed by semiquantitive RT-PCR and Southern blotting.Proliferation index was measured by MTT colorimetry.Results IL-8 levels,mRNA expression and proliferation index were significantl y higher in psoriatic patients than those in control group(P<0.05).IL-8 production and proliferation index of keratinocytes were significantly h igher in TNF-αtreated group than those in control group(P<0.05).No significant difference was found in IL-8 production and keratinocyte proliferation between sTNFRⅠ/anti-TNFRⅠ/TNF-αtreated group and control group.Conclusions Activated keratinocytes secrete ab undant cytokines crucial to the development of psoriatic lesions.By binding to TNFRⅠrather than TNFRⅡon the membrane surface,TNF-α enhances IL-8 production and proliferation of keratinocytes,and sTNFRⅠpartly neutralizes functions of TNF-α.

Key words: Psoriasis, Tumor necrosis factor, Interleukin-8, Keratinocyte