中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (3): 173-174.

• 论著 • 上一篇    下一篇

复方中药对小鼠B-16黑素瘤细胞株黑素合成和酪氨酸酶的激活作用

程东庆, 尉晓冬, 王遂泉, 许爱娥   

  1. 杭州市第三人民医院皮肤科 310009
  • 收稿日期:1999-05-04 出版日期:2000-06-15 发布日期:2000-06-15
  • 基金资助:
    中华医学会杨森基金

Effect of Traditional Chinese Medicine on the Tyrosinase Activity and Melanogenesis in B-16 Murine Melanoma

CHENG Dongqing, WEI Xiaodong, WANG Suiquan   

  1. Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou 310009
  • Received:1999-05-04 Online:2000-06-15 Published:2000-06-15

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摘要： 目的 研究复方中药对小鼠B-16黑素瘤细胞株细胞增殖、黑素合成的影响,及其对细胞内酪氨酸酶的激活作用.方法 四甲基偶氮唑蓝(MTT)比色法测定中药对细胞增殖的影响;酶学方法研究中药对酪氨酸酶活性的作用;470nm比色标准曲线法测定黑素含量.结果 研究发现复方3号(补肝肾为主)促进黑素细胞增殖和色素合成的能力高于复方1号(光敏感性药物)或复方2号(活血化瘀),而酶激活能力复方1号较为稳定.结论 在白癜风治疗中补肝肾类中药不可忽视.同时也说明影响黑素合成的因素是多方面的,可能不仅仅与酪氨酸酶活性有关.

关键词: 医学,中国传统, 酪氨酸酶, 黑色素瘤,实验性, 白癜风

Abstract: Objective To study the effect of traditional Chinese medicine on tyrosinase activity and the melanogenesis of B-16 murine melanoma cell.Methods MTT was used to study the proliferation of B-16 murine melanoma cell.The tyrosinase activity was measured by enzyme-kinetic-method.The melanin was detected by its absorption at 470nm against the standard concentration curve.Results The complex prescription 3(for invigorating liver and kidney) had a strong potential to promote the proliferation and melanogenesis of B-16 cell than complex prescription 1 (light sensitive drugs) or complex prescription 2 (for improvement of blood circulation),whereas complex prescription 1 was more stable in promoting tyrosinase activity among these remedies.Conclusion The remedies for invigorating of liver and kidney can not be neglected in vitiligo treatment.

Key words: Medicine,Chinese traditional, Tyrosine, Melanoma,experimental, Vitiligo