中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (6): 381-382.

• 论著 • 上一篇    下一篇

天疱疮抗体结合靶抗原的定位研究

杨春俊, 张学军, 杨森, 王培光, 王再兴, 刘江波   

  1. 安徽医科大学第一附属医院皮肤科 合肥 230022
  • 收稿日期:1999-02-25 修回日期:1999-05-31 出版日期:1999-12-15 发布日期:1999-12-15
  • 基金资助:
    安徽省自然科学基金(97424005)

The Location of Binding Sites of Pemphigus Antibodies with Target Antigens

YANC Chunjun, ZHANC Xuejun, YANC Sen   

  1. Department of Dermatology First Hospital of Anhui Medical Unirersity, Hefei 230022
  • Received:1999-02-25 Revised:1999-05-31 Online:1999-12-15 Published:1999-12-15

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摘要： 目的 研究天疱疮抗体结合表皮细胞间抗原在超微结构水平的部位.方法 采用LR White树脂为包埋剂,用金标记包埋后直接和间接免疫电镜技术,观察天疱疮患者皮损中IgG的沉积部位和患者血清中IgG型自身抗体结合正常人皮肤的部位.结果 寻常型天疱疮和落叶型天疱疮的直接和间接免疫电镜均在表皮细胞间的桥粒部位见金颗粒沉积,在非桥粒部位的角质形成细胞间未见金颗粒沉积.结论 寻常型天疱疮和落叶型天疱疮的靶抗原均是桥粒成分,均位于桥粒复合体上.

关键词: 天疱疮, 自身抗体, 抗原

Abstract: Objective To explore the location of binding sites of autoantibodies in patients with pemphigus.Methods The direct and indirect immunogold labeling electron microscopic techniques were used to detect the ultrastructural location of the immunoglobulin G in the skin lesions of five patients(pemphigus vulgaris 4, pemphigus foliaceus 1), and location of binding sites of the circulating autoantibodies with normal human skin in the sera of eight patients(pemphigus vulgaris 6, pemphigus foliaceus 2), using LR White resin as embedding reagent.Results Binding of gold labeling anti-human IgC was observed predominantly on desmosomal complex, but not on non-desmosomal cell surface of keratinocytes.Conclusion The target antigens of pemphigus vulgaris and pemphigus foliaceus are components of desmosome, and these antigens are located on the desmosomal complex.

Key words: Pemphigus, Autoantibodies, Antigens