中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (4): 218-220.

• 论著 • 上一篇    下一篇

间接ELISA和ABC-ELISA法对天疱疮抗体检测的研究

杨卫兵1, 刁庆春2, 叶庆佾2   

  1. 1. 解放军第一八一医院皮肤科 广西桂林 541002;
    2. 重庆第三军医大学西南医院皮肤科
  • 收稿日期:1997-08-29 修回日期:1997-12-25 出版日期:1998-08-15 发布日期:1998-08-15

Detection of Pemphigus Antibodies with Indirect ELISA and ABC-ELISA

Yang Weibing1, Diao Qingchun2, Ye Qingyi2   

  1. Department of Dermatology, 181 Hospital, Guilin, Guangxi, 541002
  • Received:1997-08-29 Revised:1997-12-25 Online:1998-08-15 Published:1998-08-15

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摘要： 目的 建立一种新的血清中的天疱疮抗体(PAb)检测方法.方法 利用PI1天疱疮抗独特型单抗,用亲和层析法纯化患者血清中的PAb;通过间接ELISA法和ABC-ELISA法建立一种PAb的ELISA检测体系.结果 纯化的PAb为IgG4亚类,间接ELISA和ABC-ELISA法检测患者血清中PAb的敏感性和特异性较高,与间接免疫荧光法比较无显着性差异,并初步得到了检测纯化PAb浓度的标准曲线.结论 本研究建立的ELISA法是一种较好的PAb定性分析法,对天疱疮的诊断具有较好的临床应用前景,并为进一步对患者血清中PAb进行定量分析打下了基础;本检测体系针对于PAb的IgG4亚类,为进一步研究IgG4在天疱疮发病机理中的意义提供了一种较好的方法.

关键词: 天疱疮, 抗体, 抗独特型, 酶联免疫吸附测定

Abstract: Objective To establish a new method for detecting pemphigus antibody (PAb). Methods PI 1 anti idiotype monoclonal antibodies against pemphigus and purified PAb from the serum of a patient with active pemphigus were used to establish an ELISA system for detecting the PAb. Results The result showed the purified PAb was IgG4 subclass, the sensitivity and specificity for the detection of PAb were high in the ELISA system, the sensitivity and specificity were not significantly different among the IIF, indirect ELISA and ABC-ELISA. The standard curve for detecting the concentration of the purified PAb was primarily obtained in the study. Conclusion The ELISA system for the detection of PAb in the sera of patients is a good qualitative method, it might be of value in the clinical diagnosis of pemphigus. It is expected to quantitatively detect PAb of pemphigus patients in the future with the ELISA system established, which is directed to the IgG4 subclass of PAb, so it may be of value in the study of IgG4 subclass in the pathogenesis of pemphigus.

Key words: Pemphigus, Antibodies,anti-idiotypic, Enzyme-linked immunosorbent assay