中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (4): 221-223.

• 论著 • 上一篇    下一篇

电镜及原子力显微镜观察生殖支原体形态学的初步探讨

骆丹1, 顾宁2, 梁国钧3, 徐文严3, 朱文元1   

  1. 1. 南京医科大学第一附属医院皮肤科 210029;
    2. 东南大学国家教委重点分子电子学实验室;
    3. 中国医学科学院皮肤病研究所
  • 收稿日期:1997-10-05 修回日期:1998-03-04 出版日期:1998-08-15 发布日期:1998-08-15

Preliminary Observation of Mycoplasma Genitalium under Electron Microscopy and Atomic Force Microscopy

Luo Dan1, Gu Ning2, Liang Guojun3   

  1. Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029
  • Received:1997-10-05 Revised:1998-03-04 Online:1998-08-15 Published:1998-08-15

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摘要： 目的 为了对初步分离成功的生殖支原体进行进一步鉴定.方法 用电子显微镜及原子力显微镜对生殖支原体标准株及分离株进行形态学的初步观察.将标本固定于云母上,在接触及轻敲模式下扫描成像,操作在常温常压下进行.结果和结论 电镜下生殖支原体多呈烧瓶状或鸭梨形,有突出的颈部及膨大的头端.原子力显微镜观察结果与之类似,典型者多呈烧瓶状或鸭梨形,颈体部多有明显缩窄环或切痕,其生殖支原体大小亦与电镜测量结果类似.

关键词: 支原体属, 显微镜检查, 电子,原子力

Abstract: Objective To further identify the isolated Mycoplasma genitalium (Mg) from the high risk population of STD in China. Mothods The morphology of Mg standard strains and clinical isolates were observed under electron microscopy (EM) and atomic force microscopy (AFM). Mg sample preparation for AFM was fixed on mica and AFM images of scanning observation were captured by contacting and tapping modes and operated under normal atmospheric pressure and temperature. Results and Conclusion Mg had a diversity of shapes, including flask, pear, spindle or round shape with projecting neck portion and slightly broadened terminal structure under EM. The morphologic features under AFM were similar to those observed by EM. The typical Mg showed shapes of flask and pear with noticeable narrow ring or mark in the neck portion. The size of Mg measured under EM and AFM was similar as well.

Key words: Mycoplasma, Microscopy,atomic force, Microscopy,electron