盐裂皮肤间接免疫荧光及BP180 NC16a酶联免疫吸附测定在大疱性类天疱疮诊断中的意义

doi:10.3760/cma.j.issn.0412-4030.2018.01.003

中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (1): 10-13.doi: 10.3760/cma.j.issn.0412-4030.2018.01.003

盐裂皮肤间接免疫荧光及BP180 NC16a酶联免疫吸附测定在大疱性类天疱疮诊断中的意义

李锁¹,李志量¹,荆可¹,向睿宇¹,张寒梅¹,冯素英²

1. 中国医学科学院皮肤病研究所
2. 中国医学科学院皮肤病研究所皮肤病医院

收稿日期:2017-09-18 修回日期:2017-10-17 出版日期:2018-01-15 发布日期:2018-01-11
通讯作者: 冯素英 E-mail:fengsuying2010@aliyun.com
基金资助:
中央级公益性科研院所基本科研业务费;北京协和医学院协和青年基金;江苏省自然科学基金;中国医学科学院医学与健康科技创新工程

Indirect immunofluorescence on salt-split skin and bullous pemphigoid 180 NC16a enzyme-linked immunosorbent assay in the diagnosis of bullous pemphigoid

Received:2017-09-18 Revised:2017-10-17 Online:2018-01-15 Published:2018-01-11
Supported by:
Special Fund for Basic Scientific Research Business of Central Public Research Institutes;PUMC Youth Fund;Natural Science Foundation of Jiangsu Province of China

摘要/Abstract

摘要：

目的探讨盐裂皮肤间接免疫荧光及大疱性类天疱疮（BP）180 NC16a-酶联免疫吸附测定（ELISA）检测在BP诊断中的意义。方法收集2015年1月至2017年8月在中国医学科学院皮肤病医院用盐裂皮肤间接免疫荧光（IIF?SSS）和BP180 NC16a?ELISA检测BP患者174例和对照组129例血清。其中25例BP患者用直接免疫荧光（DIF）进行检测并与IIF?SSS和BP180 NC16a?ELISA敏感性进行比较。结果 IIF?SSS、BP180 NC16a?ELISA的敏感性分别为93.67%、96.55%；特异性分别为100%、96.12%。IIF?SSS与BP180 NC16a?ELISA相关系数0.147，为弱相关。其中25例BP患者血清学诊断方法（IIF?SSS，BP180 NC16a?ELISA）和DIF敏感性比较差异无统计学意义。结论 BP血清学诊断方法特异性强、敏感性高，值得临床推广应用。

Abstract:

Li Suo, Li Zhiliang, Jing Ke, Xiang Ruiyu, Zhang Hanmei, Feng Suying Department of Dermatology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing 210042, China Corresponding author: Feng Suying, Email: fengsy@pumcderm.cams.cn 【Abstract】 Objective To evaluate the value of indirect immunofluorescence on salt-split skin （IIF-SSS）and bullous pemphigoid 180 NC16a enzyme-linked immunosorbent assay （BP180 NC16a-ELISA） in the diagnosis of bullous pemphigoid（BP）. Methods Serum samples were collected from 174 BP patients and 129 controls, who were enrolled from Institute of Dermatology of Chinese Academy of Medical Sciences and Peking Union Medical College between January 2015 and August 2017, and subjected to IIF-SSS and BP180 NC16a-ELISA. Direct immunofluorescence （DIF） test was performed in 25 cases of BP, and its sensitivity for the diagnosis of BP was compared with that of IIF-SSS and BP180 NC16a-ELISA. Results The sensitivities for IIF-SSS and BP180 NC16a-ELISA were 93.67% and 96.55% respectively, and the specificities for IIF-SSS and BP180 NC16a-ELISA were 100% and 96.12% respectively. IIF-SSS was weakly correlated with BP180 NC16a-ELISA with a correlation coefficient of 0.147. There was no significant difference in the sensitivity between the serological diagnostic methods （IIF-SSS and BP180 NC16a-ELISA） and DIF. Conclusion Serological diagnostic methods show high specificity and sensitivity in the diagnosis of BP, and are worthy of clinical promotion and application.

李锁李志量荆可向睿宇张寒梅冯素英. 盐裂皮肤间接免疫荧光及BP180 NC16a酶联免疫吸附测定在大疱性类天疱疮诊断中的意义[J]. 中华皮肤科杂志, 2018, 51(1): 10-13.

参考文献 12

［1］	Schmidt E, Zillikens D. Pemphigoid diseases［J］. Lancet, 2013, 381（9863）: 320⁃332. DOI: 10.1016/S0140⁃6736（12）61140⁃4.<br />
［2］	della TR, Combescure C, Cortés B, et al. Clinical presentation and diagnostic delay in bullous pemphigoid: a prospective nationwide cohort［J］. Br J Dermatol, 2012, 167（5）: 1111⁃1117. DOI: 10.1111/ j.1365⁃2133.2012.11108.x.<br />
［3］	Hofmann S, Thoma⁃Uszynski S, Hunziker T, et al. Severity and phenotype of bullous pemphigoid relate to autoantibody profile against the NH2⁃ and COOH⁃terminal regions of the BP180 ectodomain［J］. J Invest Dermatol, 2002, 119（5）: 1065⁃1073. DOI: 10.1046/j.1523⁃1747.2002.19529.x.<br />
［4］	Venning VA, Taghipour K, Mohd MMF, et al. British Association of Dermatologists′ guidelines for the management of bullous pemphigoid 2012［J］. Br J Dermatol, 2012, 167（6）: 1200⁃1214. DOI: 10.1111/bjd.12072.<br />
［5］	中国医师协会皮肤科医师分会自身免疫性疾病亚专业委员. 大疱性类天疱疮诊断和治疗的专家建议［J］. 中华皮肤科杂志, 2016, 49（6）: 384⁃387. DOI: 10.3760/cma.j.issn.0412⁃4030. 2016.06.003.<br />
［6］	de Vega IF, Iranzo⁃Fernández P, Mascaró⁃Galy JM. Bullous pemphigoid: clinical practice guidelines［J］. Actas Dermosifiliogr, 2014, 105（4）: 328⁃346. DOI: 10.1016/j.ad.2012.10.022.<br />
［7］	Nishie W. Update on the pathogenesis of bullous pemphigoid: an autoantibody⁃mediated blistering disease targeting collagen ⅩⅦ［J］. J Dermatol Sci, 2014, 73（3）: 179⁃186. DOI: 10.1016/j.jdermsci.2013.12.001.<br />
［8］	Cozzani E, Gasparini G, Burlando M, et al. Atypical presentations of bullous pemphigoid: clinical and immunopathological aspects［J］. Autoimmun Rev, 2015, 14（5）: 438⁃445. DOI: 10.1016/j.autrev.2015.01.006.<br />
［9］	Zhao CY, Murrell DF. Advances in understanding and managing bullous pemphigoid［J］. F1000Res, 2015, 4. DOI: 10.12688/f1000research.6896.1.<br />
［10］	Sárdy M, Kostaki D, Varga R, et al. Comparative study of direct and indirect immunofluorescence and of bullous pemphigoid 180 and 230 enzyme⁃linked immunosorbent assays for diagnosis of bullous pemphigoid［J］. J Am Acad Dermatol, 2013, 69（5）: 748⁃753. DOI: 10.1016/j.jaad.2013.07.009.<br />
［11］	Xu HH, Werth VP, Parisi E, et al. Mucous membrane pemphigoid［J］. Dent Clin North Am, 2013, 57（4）: 611⁃630. DOI: 10.1016/j.cden.2013.07.003.<br />
［12］	Feng S, Lin L, Jin P, et al. Role of BP180NC16a⁃enzyme⁃linked immunosorbent assay （ELISA） in the diagnosis of bullous pemphigoid in China［J］. Int J Dermatol, 2008, 47（1）: 24⁃28. DOI: 10.1111/j.1365⁃4632.2007.03531.x.<br />

盐裂皮肤间接免疫荧光及BP180 NC16a酶联免疫吸附测定在大疱性类天疱疮诊断中的意义

Indirect immunofluorescence on salt-split skin and bullous pemphigoid 180 NC16a enzyme-linked immunosorbent assay in the diagnosis of bullous pemphigoid

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