中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (2): 105-107.

• 论著 • 上一篇    下一篇

PUVA照射与青黛丸口服对银屑病患者血cAMP、cGMP、SOD及LPO含量的影响

骆丹1, 徐文严1, 李锡林2, 王书奎3, 王自正3, 蒋仲元2   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042;
    2. 南京铁道医学院附属医院;
    3. 南京放射免疫检测中心
  • 收稿日期:1996-08-16 修回日期:1996-12-10 出版日期:1997-04-15 发布日期:1997-04-15

The Effect of PUVA and Qing Dai Pill on SOD, LPO, cAMP and cGMP Levels in Psoriatic Patients

Luo Dan1, Xu Wenyan1, Li Xilin2   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042
  • Received:1996-08-16 Revised:1996-12-10 Online:1997-04-15 Published:1997-04-15

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摘要： 目的:研究PUVA及青黛丸口服治疗寻常型银屑病对患者血液中SOD活力、LPO水平、cAMP及cGMP含量的影响.方法:寻常型银屑病患者74例,随机分成PUVA照射组(36例)及青黛丸服用组(38例),按常规行治疗方案.采用微量指血超氧化物歧化酶(SOD)快速测定法测定红细胞内SOD活力,荧光分光光度法测定血清脂质过氧化物(LPO)值、放射免疫分析法测定血浆cAMP及cGMP含量.结果:治疗前患者SOD活力皆低于对照组(P<0.01),LPO水平无明显差异,cAMP低于对照组,而cGMP高于对照组.青黛丸口服后,SOD活力改善,cAMP有所回升,但LPO及cGMP变化不明显;PUVA照射后,SOD活力进一步低于疗前水平,LPO值则高于疗前,cAMP的改善略优于青黛丸,cGMP明显改善.对两种疗法的疗效、改变上述指标的可能作用机理及其与临床应用的相互关系作了分析.

关键词: 银屑病, PUVA疗法, 青黛丸, 超氧化物歧化酶类, 过氧化脂质类, 腺苷环一磷酸, 鸟苷环一磷酸

Abstract: The study compares the effect of PUVA and Qing Dai pill (a Chinese traditional medicine) regimen on SOD, LPO, cAMP and cGMP level variation in psoriatic patients. Seventy-four patients with psoriasis vulgaris were classified into two treatment groups——PUVA (36 cases) and Qing Dai pill (38 cases). SOD activity in RBC was detected by rapid catechol assay with microvolume of finger blood; serum LPO level was determined by TBA fluorescence colorimetric assay; plasma cAMP and cGMP concentrations were measured by RIA technology. Before treatment SOD activity was lower in all patients in comparison with normal controls (P<0.01). LPO level.was in normal range. cAMP was lower and cGMP higher than that of control group. After Qing Dai pill treatment, SOD activity and cAMP were improved to some degree, but LPO and cGMP levels did not show noticeable changes. After PUVA treatment, SOD activity further decreased and LPO increased. The therapeutic effect of PUVA on cAMP was a little better, but cGMP, much better than those of Qing Dai pill. The therapeutic effects of two treatments, possible mechanism of the effects on these biochemical indexes and their relation in clinical practice were discussed and analysed.

Key words: Psoriasis, PUVA, Qing Dai pill, Superoxide dismutase, Lipid peroxides, Adenosine cyclic monophosphate, Guanosine cyclic monophosphate