中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (1): 21-23.

• 论著 • 上一篇    下一篇

黑素生成素对黑素细胞增殖和黑素合成作用的研究

许爱娥1, 尉晓冬1, 毕志刚2, 陈华群2, 周渭珩1, 李一沧1   

  1. 1. 杭州第三人民医院皮肤科, 310009;
    2. 江苏省人民医院
  • 收稿日期:1996-02-17 修回日期:1996-07-17 出版日期:1997-02-15 发布日期:1997-02-15

Study of the Effects of Melagenine on Melanocyte Proliferation and Malanin Synthesis

Xu Aie1, Wei Xiaodong1, Bi Zhigang2   

  1. Department of Dermatology, Third People's Hospital, Hangzhou 310009
  • Received:1996-02-17 Revised:1996-07-17 Online:1997-02-15 Published:1997-02-15

PDF (PC)

388

赞

摘要： 古巴学者Cao报道胎盘中提取的黑素生成素(Melagenine)临床治疗白癜风有效.为了探讨治疗机理,我们按Cao法提取黑素生成素,用体外细胞培养方法,在黑素细胞培养中分别加入黑素生成素和胎盘肽,以不加黑素生成素的黑素细胞培养作为对照,用MTT法测定细胞增殖情况;用Oikawa法测定黑素合成量;用透射电镜观察黑素小体的生成.结果表明,加黑素生成素后,黑素细胞促增殖率为42.5%,加胎盘肽促增殖率为5.9%,二者有显著性差异(P<0.05);加黑素生成素后黑素合成量为5.5fg/细胞,对照组为4.5fg/细胞,透射电镜观察显示加黑素生成素后黑素小体增多.本研究从体外证明了黑素生成素能促进黑素细胞增殖,促进黑素的合成,这可能是临床治疗有效的机理.

关键词: 黑素生成素, 黑素细胞, 黑素, 白癜风

Abstract: Cao reported that melagenine extracted from human placenta was effective in the clinical treatment of vitiligo, to elucidate the possible mechanism involved, melagenine and placenta peptide were added to melanocytes in the culture, and the culture without melagenine as control. The results obtained by MTT showed that melagenine can stimulate melanoeyte proliferation, its promoting rate was 42.5%, while placenta peptide got a promoting rate of 5.9%, the difference was significant (P<0.05). The method of Oikawa was employed to measure the melanin content of melanocytes. As a resuit, cells cultured with melagenine had a melanin content of 5.5fg/cell and control cells 4.5fg/cell on average. Observation of cell ultrastrueture by transmitted electron microscope demonstrated that ceils cultured with melagenine had more melanosomes than those of control. This study proves that melagenine can stimulate melanocyte proliferation and melanin synthesis, which may be the mechanism that make it valid in clinical treatment.

Key words: Melagenine, Melanocyte, Melanin, Vitiligo