中华皮肤科杂志 ›› 1995, Vol. 28 ›› Issue (2): 67-69.

• 论著 •    下一篇

银屑病患者皮损表皮生长因子及其受体的检测

刘宝军1, 张海涛1, 李淑琴1, 陈伟2, 李润江1   

  1. 1. 沈阳军区总医院皮肤科, 110015;
    2. 沈阳军区总医院医学实验科
  • 收稿日期:1994-04-12 修回日期:1994-11-05 出版日期:1995-04-15 发布日期:1995-04-15

Immunolocalization of Epidermal Growth Factor and Epidermal Growth Factor Receptors in Psoriatic Epidermis

Liu Baojun1, Zhang Haitao1, Li Shuqin1   

  1. Shenyang Military General Hospital, Shenyang 110015
  • Received:1994-04-12 Revised:1994-11-05 Online:1995-04-15 Published:1995-04-15

摘要: 用抗表皮生长因子(EGF)多克隆抗体、抗表皮生长因子受体(EGFR)单克隆抗体及ABC免疫酶标技术,对20例寻常型银屑病患者皮损及10例正常人皮肤进行观察.结果表明:①正常人皮肤及银屑病非皮损区皮肤EGF及EGFR主要分布在表皮基底细胞层及基底层上部.②银屑病进行期皮损EGF及EGFR分布于表皮各层,表皮中、上层含量明显升高.③经有效治疗消退期皮损EGF及EGFR从角质层开始消退,分布趋于正常.提示EGF及EGFR对银屑病皮损角肮细胞过度增殖及异常分化起重要作用.

关键词: 银屑病, 表皮生长因子, 受体,表皮生长因子

Abstract: We used an avidin-biotin-complex immunoperoxidase technique with anti-epidermal growth factor (EGF) polyclonal antibody and anti-EGF receptors (EGFR) monoclonal antibody to investigate the skin lesions from 20 patients with psoriasis vulgaris and normal skin from 10 healthy volunteers.Our results showed that: (1) The immunoreactive proteins of EGF and EGFR were primarily restricted to the hasal cells and suprabasal keratinocytes in both normal epidermis and uninvolved psoriatic epidermis; (2) EGF and EGFR were distributed in all epidermal layers of active psoriatic lesions, and elevated levels of EGF and EGFR were seen in the mid and upper epidermal layers; (3) The immunoreactive proteins of EGF and EGFR were absent in the stratum corneum and decreased in the upper epidermal layers in regressing psoriatic lesions.It is suggested that EGF and EGFR may play an important role in the hyperproliferation and abnormal differentiation of keratinocytes seen in psoriasis.

Key words: Psoriasis, Epidermal growth factor, Receptors, epidermal growth factor