特应性皮炎的转录组学研究进展

doi:10.35541/cjd.20210333

中华皮肤科杂志 ›› 2022, Vol. 55 ›› Issue (4): 365-369.doi: 10.35541/cjd.20210333

特应性皮炎的转录组学研究进展

覃诗纯李梦杰陆晓云肖风丽

安徽医科大学第一附属医院皮肤性病科安徽医科大学皮肤病研究所，合肥 230022

收稿日期:2021-04-23 修回日期:2021-10-27 发布日期:2022-04-01
通讯作者: 肖风丽 E-mail:xiaofengli@126.com
基金资助:
国家自然科学基金（81972926）；安徽省学术和科技带头人科研活动经费资助项目（2017D141）

Transcriptomics in atopic dermatitis

Qin Shichun, Li Mengjie, Lu Xiaoyun, Xiao Fengli

Department of Dermatology and Venereology, The First Affiliated Hospital of Anhui Medical University, Institute of Dermatology, Anhui Medical University, Hefei 230022, China

Received:2021-04-23 Revised:2021-10-27 Published:2022-04-01
Contact: Xiao Fengli E-mail:xiaofengli@126.com
Supported by:
National Natural Science Foundation of China （81972926）; Anhui Academic and Science and Technology Leaders Scientific Research Activity Funding Project （2017D141）

摘要/Abstract

摘要： 【摘要】随着基因芯片技术、RNA测序技术等转录组学技术发展，特应性皮炎（AD）发病中重要的相关影响因素逐渐被揭示，如不同T辅助（Th）细胞的亚型以及其他免疫相关细胞如巨噬细胞、朗格汉斯细胞；在AD的瘙痒及皮肤屏障破坏方面，相关免疫细胞如Th2细胞及角质形成细胞等所释放白细胞介素4、白细胞介素13、聚丝蛋白、兜甲蛋白等活性物质的异常变化起着主要作用。同时，转录组技术已被用于分析患者治疗前后转录谱的变化从而对患者的病情和治疗效果进行评估等。本文总结近年来在AD转录组学方面的研究进展。

关键词: 皮炎, 特应性, 转录组, 芯片分析技术, RNA测序技术

Abstract: 【Abstract】 With the development of transcriptomic technologies such as gene chip technology and RNA sequencing technology, important related factors in the pathogenesis of atopic dermatitis （AD） have been gradually identified, such as different T helper （Th） cell subtypes and other immune-related cells （macrophages and Langerhans cells）; abnormal changes in active substances such as interleukin-4, interleukin-13, fillagrin and loricrin released by immune-related cells such as Th2 cells and keratinocytes have been found to play major roles in pruritus and skin barrier damage in AD. In recent years, transcriptomic technologies have been applied to the analysis of changes in transcriptomic profiles of patients before and after treatment to evaluate patients′ condition and therapeutic effect. This review summarizes research progress in transcriptomics in AD in recent years.

Key words: Atopic dermatitis, Transcriptome, Microchip analytical procedures, RNA sequencing

覃诗纯李梦杰陆晓云肖风丽. 特应性皮炎的转录组学研究进展[J]. 中华皮肤科杂志, 2022,55(4):365-369. doi:10.35541/cjd.20210333

Qin Shichun, Li Mengjie, Lu Xiaoyun, Xiao Fengli. Transcriptomics in atopic dermatitis[J]. Chinese Journal of Dermatology, 2022, 55(4): 365-369.doi:10.35541/cjd.20210333

参考文献 36

［1］	Brown SJ, Elias MS, Bradley M. Genetics in atopic dermatitis: historical perspective and future prospects［J］. Acta Derm Venereol, 2020,100（12）:adv00163. doi: 10.2340/00015555⁃3513.
［2］	Mucha S, Baurecht H, Novak N, et al. Protein⁃coding variants contribute to the risk of atopic dermatitis and skin⁃specific gene expression［J］. J Allergy Clin Immunol, 2020,145（4）:1208⁃1218. doi: 10.1016/j.jaci.2019.10.030.
［3］	Besin G, Yousefi M, Saba I, et al. Dok⁃1 overexpression promotes development of γδ natural killer T cells［J］. Eur J Immunol, 2012,42（9）:2491⁃2504. doi: 10.1002/eji.201242421
［4］	Chiricozzi A, Maurelli M, Peris K, et al. Targeting IL⁃4 for the treatment of atopic dermatitis［J］. Immunotargets Ther, 2020,9:151⁃156. doi: 10.2147/ITT.S260370.
［5］	Majewska A, Gajewska M, Dembele K, et al. Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis［J］. BMC Vet Res, 2016,12（1）:174. doi: 10.1186/s12917⁃016⁃0805⁃6.
［6］	Castro G, Liu X, Ngo K, et al. RORγt and RORα signature genes in human Th17 cells［J］. PLoS One, 2017,12（8）:e0181868. doi: 10.1371/journal.pone.0181868.
［7］	Ha Y, Lee WH, Jeong J, et al. Pyropia yezoensis extract suppresses IFN⁃gamma⁃ and TNF⁃alpha⁃induced proinflammatory chemokine production in HaCaT cells via the down⁃regulation of NF⁃κB［J］. Nutrients, 2020,12（5）:1238. doi: 10.3390/nu12051238.
［8］	Shin KO, Ha DH, Kim JO, et al. Exosomes from human adipose tissue⁃derived mesenchymal stem cells promote epidermal barrier repair by inducing de Novo synthesis of ceramides in atopic dermatitis［J］. Cells, 2020,9（3）:680. doi: 10.3390/cells 9030680.
［9］	Nedoszytko B, Reszka E, Gutowska⁃Owsiak D, et al. Genetic and epigenetic aspects of atopic dermatitis［J］. Int J Mol Sci, 2020,21（18）:6484. doi: 10.3390/ijms21186484.
［10］	Fujii M. Current understanding of pathophysiological mechanisms of atopic dermatitis: interactions among skin barrier dysfunction, immune abnormalities and pruritus［J］. Biol Pharm Bull, 2020,43（1）:12⁃19. doi: 10.1248/bpb.b19⁃00088.
［11］	Freudenberg JM, Olivry T, Mayhew DN, et al. The comparison of skin transcriptomes confirms canine atopic dermatitis is a natural homologue to the human disease［J］. J Invest Dermatol, 2019,139（4）:968⁃971. doi: 10.1016/j.jid.2018.10.018.
［12］	Pavel AB, Renert⁃Yuval Y, Wu J, et al. Tape strips from early⁃onset pediatric atopic dermatitis highlight disease abnormalities in nonlesional skin［J］. Allergy, 2021,76（1）:314⁃325. doi: 10. 1111/all.14490.
［13］	Torres T, Ferreira EO, Gonçalo M, et al. Update on atopic dermatitis［J］. Acta Med Port, 2019,32（9）:606⁃613. doi: 10. 20344/amp.11963.
［14］	Lee YS, Han SB, Ham HJ, et al. IL⁃32γ suppressed atopic dermatitis through inhibition of miR⁃205 expression via inactivation of nuclear factor⁃kappa B［J］. J Allergy Clin Immunol, 2020,146（1）:156⁃168. doi: 10.1016/j.jaci.2019.12.905.
［15］	Dyjack N, Goleva E, Rios C, et al. Minimally invasive skin tape strip RNA sequencing identifies novel characteristics of the type 2⁃high atopic dermatitis disease endotype［J］. J Allergy Clin Immunol, 2018,141（4）:1298⁃1309. doi: 10.1016/j.jaci.2017.10. 046.
［16］	Furue M. Regulation of filaggrin, loricrin, and involucrin by IL⁃4, IL⁃13, IL⁃17A, IL⁃22, AHR, and NRF2: pathogenic implications in atopic dermatitis［J］. Int J Mol Sci, 2020,21（15）:5382. doi: 10.3390/ijms21155382.
［17］	Bieber T. Interleukin⁃13: targeting an underestimated cytokine in atopic dermatitis［J］. Allergy, 2020,75（1）:54⁃62. doi: 10.1111/ all.13954.
［18］	Rajesh A, Wise L, Hibma M. The role of Langerhans cells in pathologies of the skin［J］. Immunol Cell Biol, 2019,97（8）:700⁃713. doi: 10.1111/imcb.12253.
［19］	Huang L, Li GH, Yu Q, et al. Smad2/4 signaling pathway is critical for epidermal langerhans cell repopulation under inflammatory condition but not required for their homeostasis at steady state［J］. Front Immunol, 2020,11:912. doi: 10.3389/fimmu.2020.00912.
［20］	Brunner PM, Israel A, Leonard A, et al. Distinct transcriptomic profiles of early⁃onset atopic dermatitis in blood and skin of pediatric patients［J］. Ann Allergy Asthma Immunol, 2019,122（3）:318⁃330.e3. doi: 10.1016/j.anai.2018.11.025.
［21］	Novak H, Müller A, Harrer N, et al. CCL23 expression is induced by IL⁃4 in a STAT6⁃dependent fashion［J］. J Immunol, 2007,178（7）:4335⁃4341. doi: 10.4049/jimmunol.178.7.4335.
［22］	Devos M, Mogilenko DA, Fleury S, et al. Keratinocyte expression of A20/TNFAIP3 controls skin inflammation associated with atopic dermatitis and psoriasis［J］. J Invest Dermatol, 2019,139（1）:135⁃145. doi: 10.1016/j.jid.2018.06.191.
［23］	Yuan W, Chen Y, Zhou Y, et al. Formononetin attenuates atopic dermatitis by upregulating A20 expression via activation of G protein⁃coupled estrogen receptor［J］. J Ethnopharmacol, 2021,266:113397. doi: 10.1016/j.jep.2020.113397.
［24］	Nattkemper LA, Tey HL, Valdes⁃Rodriguez R, et al. The genetics of chronic itch: gene expression in the skin of patients with atopic dermatitis and psoriasis with severe itch［J］. J Invest Dermatol, 2018,138（6）:1311⁃1317. doi: 10.1016/j.jid.2017.12. 029.
［25］	Tham EH, Dyjack N, Kim BE, et al. Expression and function of the ectopic olfactory receptor OR10G7 in patients with atopic dermatitis［J］. J Allergy Clin Immunol, 2019,143（5）:1838⁃1848.e4. doi: 10.1016/j.jaci.2018.11.004.
［26］	Denda M. Newly discovered olfactory receptors in epidermal keratinocytes are associated with proliferation, migration, and re⁃epithelialization of keratinocytes［J］. J Invest Dermatol, 2014,134（11）:2677⁃2679. doi: 10.1038/jid.2014.229.
［27］	Aida Maranduca M, Liliana Hurjui L, Constantin Branisteanu D, et al. Skin ⁃ a vast organ with immunological function （Review）［J］. Exp Ther Med, 2020,20（1）:18⁃23. doi: 10.3892/etm.2020. 8619.
［28］	Spidale NA, Malhotra N, Frascoli M, et al. Neonatal⁃derived IL⁃17 producing dermal γδ T cells are required to prevent spontaneous atopic dermatitis［J］. Elife, 2020,9:e51188. doi: 10.7554/eLife.51188.
［29］	Mizutani N, Sae⁃Wong C, Kangsanant S, et al. Thymic stromal lymphopoietin⁃induced interleukin⁃17A is involved in the development of IgE⁃mediated atopic dermatitis⁃like skin lesions in mice［J］. Immunology, 2015,146（4）:568⁃581. doi: 10.1111/imm.12528.
［30］	Mirshafiey A, Simhag A, El Rouby NM, et al. T⁃helper 22 cells as a new player in chronic inflammatory skin disorders［J］. Int J Dermatol, 2015,54（8）:880⁃888. doi: 10.1111/ijd.12883.
［31］	He H, Suryawanshi H, Morozov P, et al. Single⁃cell transcriptome analysis of human skin identifies novel fibroblast subpopulation and enrichment of immune subsets in atopic dermatitis［J］. J Allergy Clin Immunol, 2020,145（6）:1615⁃1628. doi: 10.1016/j.jaci.2020.01.042.
［32］	Kennedy K, Heimall J, Spergel JM. Advances in atopic dermatitis in 2017［J］. J Allergy Clin Immunol, 2018,142（6）:1740⁃1747. doi: 10.1016/j.jaci.2018.10.012.
［33］	Guttman⁃Yassky E, Bissonnette R, Ungar B, et al. Dupilumab progressively improves systemic and cutaneous abnormalities in patients with atopic dermatitis［J］. J Allergy Clin Immunol, 2019,143（1）:155⁃172. doi: 10.1016/j.jaci.2018.08.022.
［34］	Ko KC, Tominaga M, Kamata Y, et al. Possible antipruritic mechanism of cyclosporine a in atopic dermatitis［J］. Acta Derm Venereol, 2016,96（5）:624⁃629. doi: 10.2340/00015555⁃2318.
［35］	Möbus L, Rodriguez E, Harder I, et al. Atopic dermatitis displays stable and dynamic skin transcriptome signatures［J］. J Allergy Clin Immunol, 2021,147（1）:213⁃223. doi: 10.1016/j.jaci.2020. 06.012.
［36］	Lossius AH, Berents TL, Saetre F, et al. Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL⁃36γ and IL⁃37［J］. Exp Dermatol, 2021,30（2）:249⁃261. doi: 10.1111/exd.14217.

[1]	孙晓丽李邻峰. 肥大细胞在变应性接触性皮炎发病中的作用进展[J]. 中华皮肤科杂志, 2022, 55(5): 449-451.
[2]	申晨陶娟. 特应性皮炎长期控制的评估演变与达标治疗[J]. 中华皮肤科杂志, 2022, 55(5): 442-445.
[3]	李巍尹慧彬. 特应性皮炎的部位异质性[J]. 中华皮肤科杂志, 2022, 55(4): 349-352.
[4]	李妍李明徐薇李邻峰. 【开放获取】凯普斯泰冷敷凝胶治疗成人轻中度特应性皮炎的随机双盲对照研究[J]. 中华皮肤科杂志, 2022, 55(4): 345-348.
[5]	何素玲田歆梁景耀邵蕾李俊龙黄琼霄刘玉梅王建琴. 重度特应性皮炎患者急性期和缓解期皮肤微生态研究[J]. 中华皮肤科杂志, 2022, 55(4): 329-336.
[6]	刘擘宋晓婷廖双璐于淼栾婷婷李若瑜赵作涛. 【开放获取】巴瑞替尼治疗中重度特应性皮炎的疗效和安全性观察[J]. 中华皮肤科杂志, 2022, 55(4): 304-307.
[7]	田晶梁源申春平赵牧童汪洋王珊焦磊马琳. 儿童中重度特应性皮炎首次皮下注射度普利尤单抗短期疗效和安全性分析[J]. 中华皮肤科杂志, 2022, 55(4): 299-303.
[8]	刘擘宋晓婷李若瑜赵作涛. 【开放获取】度普利尤单抗治疗特应性皮炎的疗效及安全性分析[J]. 中华皮肤科杂志, 2022, 55(4): 295-298.
[9]	中国中西医结合学会皮肤性病专业委员会环境与职业性皮肤病学组中华医学会皮肤性病学分会儿童学组. 【开放获取】特应性皮炎湿包疗法临床应用专家共识[J]. 中华皮肤科杂志, 2022, 55(4): 289-294.
[10]	中国中西医结合学会皮肤性病专业委员会环境与职业性皮肤病学组中华医学会皮肤性病学分会儿童学组中国老年保健医学研究会皮肤科分会. 特应性皮炎外用制剂合理应用及患者指导专家共识[J]. 中华皮肤科杂志, 2022, 55(4): 281-288.
[11]	王傲姚煦. 特应性皮炎瘙痒机制的研究进展[J]. 中华皮肤科杂志, 2022, 55(4): 357-361.
[12]	余愫郁天泽李巍. 自主神经在特应性皮炎免疫失调中的作用[J]. 中华皮肤科杂志, 2022, 55(4): 362-365.
[13]	宋志强顾恒. 加强皮炎湿疹类疾病诊断术语的规范化[J]. 中华皮肤科杂志, 2021, 54(8): 665-667.
[14]	杨骥徐欣植李明. 脂溢性皮炎样皮肌炎：一种特殊类型的皮肌炎[J]. 中华皮肤科杂志, 2021, 54(7): 639-641.
[15]	门月华韩慧璞艾克德·阿力甫姚义凡黄婷婷康佳蕊王铁山张明波鞠延娇初明王月丹. 2，4-二硝基氯苯诱导的特应性皮炎样小鼠模型的皮肤菌群特征研究[J]. 中华皮肤科杂志, 2021, 54(7): 605-611.

特应性皮炎的转录组学研究进展

Transcriptomics in atopic dermatitis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 36

相关文章 15

Metrics

本文评价

推荐阅读 10