聚合酶链反应Dig-dUTP掺入法制备桥粒芯蛋白1、3型探针

Chinese Journal of Dermatology ›› 1997, Vol. 30 ›› Issue (4): 248-250.

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Preparation of Desmoglein 1,3 Type Probes Using PCR of Incorporation of Dig-dUTP Method

Han Gangwen¹, Zhou Lijun², Ma Shengqing¹

Department of Dermatology, First Teaching Hospital, Beijing Medical University, Beijing 100034

Received:1996-08-20 Revised:1996-12-16 Online:1997-08-15 Published:1997-08-15

Abstract

Abstract: In this study we successfully prepared desmoglein 1 and desmoglein 3 DNA probes by incorporation of Dig-dUTP during PCR amplification. Eleetrophoresis analysis showed that Dig labelled probes moved slower on the gel than PCR products not incorporated with Dig due to the enlargement of probe fragment. The results of Dot-blot showed that the prepared probes can be used for RNA analysis, and both probes have high specificity, The results provided basis for the quantitative and qualitative study of desmoglein gene expression in tissues or cells by means of in situ hybridization and Northern hybridization etc.

Key words: Desmoglein, Probes

Han Gangwen, Zhou Lijun, Ma Shengqing. Preparation of Desmoglein 1,3 Type Probes Using PCR of Incorporation of Dig-dUTP Method[J].Chinese Journal of Dermatology, 1997, 30(4): 248-250.

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Preparation of Desmoglein 1,3 Type Probes Using PCR of Incorporation of Dig-dUTP Method

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