羟氯喹及没食子酸酯对HaCaT细胞光照射的影响

Abstract

Abstract: Objective To investigate the photo-protective mechanisms of hydroxychloroquine and epigallocatechin gallate (EGCG) on HaCaT cells damaged from UVB irradiation. Methods Subconfluent HaCaT cells were irradiated with different doses of UVB irradiation and treated with the above listed agents. The mRNA expression levels of p53, p21, c-fos and GADPH genes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Results UVB irradiation induced mRNA expression of p53, p21 and c-fos in cultured HaCaT cells, which were alleviated by hydroxychloroquine and EGCG treatment in UVB irradiation group. Conclusions The photo-protective effects of hydroxychloroquine and EGCG on HaCaT cells by UVB irradiation might be related to inhibition of the expression of p53,p21 and c-fos genes.

Key words: Ultraviolet rays, Cells, cultured, Hydroxychloroqine, Protein p53, Proto-oncogene proteins c-fos, Proto-oncogene proteins p21(ras), Epigallocatechin gallate

MIN Wei, LUO Dan, LIN Xiang-fei, WU Die, MIAO Xu. Study on Photo-protective Mechanisms of Hydroxychloroquine and Epigallocatechin Gallat on Keratinocytes Damaged from Ultraviolet-B Irradiation[J].Chinese Journal of Dermatology, 2005, 38(9): 575-577.

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Study on Photo-protective Mechanisms of Hydroxychloroquine and Epigallocatechin Gallat on Keratinocytes Damaged from Ultraviolet-B Irradiation

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