Abstract: Objective To investigate the-109 polymorphism at the promoter region of high-affinity IgE receptorβ(FceR1β)gene and its relationship with serum IgE in patients with atopic dermatitis in Han nationality in Hunan Province,China.Methods DNA was obtained from 20 patients with atopic dermatitis and 20 healthy controls.The-109 polymorphism was detected by PCR-single strand conformation polymorphism (SSCP),and confirmed by direct sequencing.ELISA was applied to measure the serum total IgE.Results Three mutations were identified in 4 of the 20 patients,including a G138A mutation and a T159C mutation in 2 patients,as well as a A165G mutation in the other 2 patients.The level of serum total IgE was 1.88±1.30 mg/L in the 4 patients with mutations,which was significantly different(P<0.05) from that (0.32±0.12 mg/L)in the 16 patients without mutations and that(0.20±0.05 mg/L)in the healthy controls.Conclusion The-109 polymorphism at the promoter region of FceRIβgene may be associated with the increase of serum IgE level.

Key words: Dermatitis, atopic, Receptors, IgE, Polymorphism, single nucleotide, Mutation, Immunoglobulin E