Abstract: Jiang Weiping*, Zhang Songtao, Jiang Huiling, Gong Wei, Wu Shaofang, Yu Xinmin, Song Xin. *Department of Clinical Laboratory, Lishui Central Hospital, Lishui 323000, Zhejiang, China Corresponding author: Jiang Weiping, Email: jwp225@126.com 【Abstract】 Objective To investigate the distribution of human papillomavirus （HPV） genotypes in male patients with anogenital warts in Lishui area, Zhejiang province. Methods Tissue specimens were obtained from the lesions of 150 male patients with anogenital warts. PCR-reverse dot blot hybridization was performed to detect the presence of 3 low-risk HPV types （HPV 6, 11, and 43） and 16 moderate- or high-risk HPV types （HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68 and CP8304） in these specimens. Chi-square test was used for statistical analysis. Results HPV was detected in 91 （60.67%） of the 150 male patients. Of the 91 positive patients, 74 （81.32%） were infected by single or multiple low-risk HPV types, whereas 17 （18.68%） by single or multiple high-risk HPV types. Thirty-one （34.07%） patients harbored 2 － 5 HPV genotypes, including 20 （64.52%） patients infected with both low-risk and high-risk HPV types, and 6 （19.35%） patients infected with two low-risk HPV types. The coexistence of moderate- or high-risk HPV types with HPV 6 was observed in 13 （41.94%） patients, and that with HPV 11 in 6 （19.35%） patients. The most prevalent genotype was HPV 6 （28.68%, 39/136）, followed by HPV 11 （26.47%, 36/136 ）, 16 （8.09%, 11/136 ）, 52 （5.15%, 7/136 ）, 53 （5.15%, 7/136 ）, 51 （4.41%, 6/136 ）, 58 （4.41%, 6/136 ） and 43 （4.41%, 6/136 ）. Conclusions Low-risk HPV genotypes predominate in male patients with anogenital warts, and there are large differences in the distribution of multiple infections and HPV genotypes.

Key words: Papillomavirus infections, Genitalia, Anus, Genotype