Chinese Journal of Dermatology ›› 2011, Vol. 44 ›› Issue (12): 894-895.

• Techniques and methods • Previous Articles     Next Articles

Development of a strategy to prevent and control the contamination with meticillin-resistant Staphylococcus epidermidis during the in vitro culture of Chlamydia trachomatis

  

  • Received:2011-03-18 Revised:2011-05-07 Online:2011-12-15 Published:2011-12-02
  • Contact: quanzhong liu E-mail:liuquanzhong@medmail.com.cn

Abstract:

Objective To investigate the method of control and prevention for Meticillin-resistant Staphylococcus epidermidis(MRSE) contamination. Methods Diffusion method and broth method to screening for susceptible antibiotics. Microdilution to determine MIC and cytotoxic effect.Results MRSE was susceptible to cefotaxime sodium, Cefoperazone and Sulbactam and ceftriaxone sodium. MICs were 50,20,100 mg/L respectively.They made the conclusion get less or small.Gentamicin and Vancomycin were susceptible,which MICs were 3200U/mL and 400mg/L ,but they had obvious cytotoxic effect. Conclusion Prevention for MRSE contamination is very important.The third genenation cephalosporin can control the MRSE,but they impact the Chlamydia trachomatis.The favorite treatment is to add the gentamicin for 1-2 hours.