Chinese Journal of Dermatology ›› 2011, Vol. 44 ›› Issue (11): 805-807.
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Abstract:
Objective To study the single nucleotide polymorphisms (SNPs) in the ORF26 gene of HHV-8 in Kaposi′s sarcoma (KS), and to assess their correlations with the clinical phenotype and mucosal invasion of KS. Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from paraffin-embedded tissue specimens obtained from 32 cases of KS (including 26 classic and 6 AIDS-related KS). The ORF26 gene of HHV-8 was amplified by nested-PCR followed by bidirectional sequencing. The software DNAStar and program Clustal W were used to assess the SNPs in the ORF26 gene. Statistical analysis was carried out by using the Fisher′s exact probability test. Results HHV-8 DNA was detected in 30 of the 32 tissue specimens, and in all of the 6 AIDS-related specimens. The predominant SNPs were 981 T/C (n = 12), 1086 C/T (n = 12) and 1139 A/C (n = 12) in the ORF26 gene of the 30 strains of HHV-8. No significant difference was observed in the distribution of SNPs in ORF26 between different phenotypes of KS or between KS with and without mucosal invasion. Conclusion The ORF26 SNPs of HHV-8 seem unrelated to the clinical phenotypes or mucosal invasion of KS.
Key words: Single nucleotide polymorphisms
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