UVB对Jurkat细胞Gadd45a表达和DNA甲基化水平的影响

Abstract

Abstract:

Objective To explore the effects of UVB on the expression of Gadd45a gene and DNA methylation levels in Jurkat cells. Methods Jurkat cells were irradiated with UVB of 1.0 J/cm2 and 1.5 J/cm2 respectively, and collected at 6, 12, 24 and 48 hours after the irradiation. Real-time RT-PCR was used to detect the mRNA expression of Gadd45a gene and methylation-sensitive genes CD11a and CD70. Global methylation level was also measured by MethylAmp global DNA methylation quantification kit. Results After irradiation with UVB at 1.0 J/cm2, the mRNA level of Gadd45a increased but global methylation level decreased at 6, 12, 24 and 48 hours, and significant changes were observed at 6 and 12 hours for the level of both Gadd45a mRNA expression and global methylation （P < 0.01 or 0.05）. Elevated mRNA expressions of CD11a and CD70 were also noted in Jurkat cells after irradiation with UVB of 1.0 J/cm2, and significant elevation was observed at 12 hours （both P < 0.05）. After irradiation with UVB of 1.5 J/cm2, there was a statistical increase in the mRNA expressions of Gadd45a, CD11a, CD70, together with a statistical decrease in global methylation level in Jurkat cells, at 6, 12, 24 and 48 hours （P < 0.01 or 0.05）. The mRNA expression of Gadd45a negatively correlated with the global level of DNA methylation in Jurkat cells （r = -0.395, P < 0.05）. Conclusion UVB irradiation can upregulate the expression of Gadd45a, but downregulate the global DNA methylation level in Jurkat cells.

Key words: 【Key words】 ultraviolet, Jurkat cells, Gadd45a, methylation

References

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Effects of ultraviolet B （UVB） on the expression of Gadd45a gene and DNA methylation level in Jurkat cells

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