Abstract:

【Abstract】Objective To compare differences in effects of different glucocorticoids on expression of glucocorticoid receptor in human keratinocyte HaCaT cell line. Methods Cultured HaCaT cells line were divided into hydrocortisone stimulation group and dexamethasone stimulation group and betamethasone stimulation group. The expressions of GRα, β mRNA and protein were examined by Reverse transcription-Real time polymerase chain reaction (RT-PCR) and Western blotting. Results The three kinds of the glucocorticoids with different efficacy could down-regulate the expression of GRα in HaCaT cells line, hydrocortisone had most poorly effect while no difference between dexamethasone with betamethasone exists. The three kinds of glucocorticoid could up-regulate the expression of GRβ in HaCaT cells line，but no difference exists among them. Conclusion The different effects on the expression of GRα and GRβ in keratinocytes by different GC could give guidance for correctly using glucocorticoids with different efficacy.

Key words: Glucocorticoid, Glucocorticoid receptor, Hydrocortisone, Dexamethasone, Betamethasone, Keratinocytes