他克莫司影响朗格汉斯细胞迁移机制的研究

Abstract

Abstract: Objective To investigate the mechanisms of tacrolimus on the migration of Langerhans;cells.Methods The concentration of MCP-1 in the cultured supernatants of HaCaT cell treated with tacrolimus was determined by ELISA.The expression level of MCP-1 mRNA in HaCaT cell treated with tacrolimus was studied by RT-PCR method.Results The concentration of MCP-1 in HaCaT cell treated with 625 ～ 5 000 ng/mL of tacrolimus was significantly decreased.The expression level of MCP-1 mRNA was also markedly decreased.Conclusions Tacrolimus may probably decrease the expression of MCP-1 in keratinocytes and suppress the chemotactic ability of Langerhans;cells precursor and Langerhans;cells,thus inhibiting or diminishing certain local immunoreaction.

Key words: Langerhans cells, Immunosuppressive agents, Monocyte chemoattractant protein-1, Cell movements

TANG Ling, YU Yi-zhi, CHEN Jie, YAN Ming, GU Jun, ZHENG Mao-rong. The Mechanisms of Tcrolimus on the Migration of Langerhans'Cells[J].Chinese Journal of Dermatology, 2004, 37(11): 653-655.

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The Mechanisms of Tcrolimus on the Migration of Langerhans'Cells

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