Abstract: Objective To investigate the biological activities of a conditioned medium for human dermal papilla.Methods Culture medium of the lower passage human dermal papilla cells was collected as the conditioned medium.The growth pattern and the growth curve of the higher passage human dermal papilla cells cultured with conditioned medium were observed in vitro.And the morphology of the co-culture of the higher passage human dermal papilla cells and the lower passage human dermal papilla cells was observed.Results The higher passage human dermal papilla cells,which was cultured with conditioned medium from the lower passage human dermal papilla cells,showed aggregative growth pattern.And the growth curve of the higher passage human dermal papilla cells was much better than that in the control groups (P<0.01),and the values of 3H-TdR incorporation were higher than those in the control groups (P<0.01).The co-cultured higher passage human dermal papilla cells with lower passage human dermal papilla cells showed aggregative growth pattern.Conclusion These results show that the lower passage human dermal papilla cells cultured in vitro might secrete some chemotactic factors that have some biological activities on the high passage human dermal papilla cells.

Key words: Culture media,conditioned, Cell culture, Hair Follicle