Abstract: Objective To detect human papillomavirus(HPV)DNA in peripheral blood of patients with condyloma acuminata(CA).Methods Polymerase chain reaction(PCR)was used to amplify the HPV DNA in skin lesions and peripheral blood of 30 patients with CA,and 20 normal blood donors as controls.Re-striction endonucleases Rsa I and Pst I were used for HPV DNA typing.The PCR products from 1 patient who was HPV positive for both skin lesions and peripheral blood,were cloned and sequenced.Results HPV DNA was identified in all CA skin lesions.HPV DNA was identified in peripheral blood mononuclear cells(PBM-Cs)from 8 of 30(26.7%)patients and in none of 20 control subjects(P<0.05).HPV DNA was not found in the plasma.In all 8 patients who had positive results for both skin lesions and PBMCs,the results of DNA sequence and endonuclease cleavage showed that the types of HPV found in the skin lesions were in accordance with those found in the PBMCs.Conclusion These findings suggest that viremia of HPV may occur in CA,and HPV DNA in PBMCs may serve as a source of virus reinfection.

Key words: Papillomavirus,human, Condylomata acuminata, Leukocytes,mononuclear