Abstract: Objective To study the immunogenicity of extractable nuclear antigens(ENA)in activateed lymphocytes.Methods The ENA of the normal and activated lymphocytes was extracted according to Sharp's method,then syngeneic BALB/C mice were immunized.The dynamic fluctuation of serum IgG anti-dsDNA antibody level in mice was analyzed by ELISA,so did the ENA polypeptide spectrum.The immunofluorescent staining pattern of ANA and renal immunopathologic changes of the mice were investigated.Results ANA could be detected in the sera of the immunized mice by the ENA extracted from the activated lymphocytes,including anti-dsDNA and anti-ENA.The immunofluorescent staining patterns for ANA manifested as homogeneous pattern,peripheral pattern,speckled pattern and nucleolar pattern.Moreover,marked immune complex deposits in glomerulus could be observed in ANA positive mice.The results in those mice immunized by the normal-lymphocyte-ENA were negative.Conclusion The ENA extracted from activated lymphocytes is immunogenic,can drive the production of ANA and cause SLE-like syndrome.

Key words: Lupus erythematosus, systemic, Antibodies, antinuclear, Autoantibodies