Abstract: Pathogenic Mycoplasma genitalium multiplies slowly and is difficult to be cultured. Furthermore, M.genitalium(Mg) and M.pneumoniae share common antigens, which can give rise to extensive cross-reactions in serological tests, this makes the diagnosis of Mg infection difficult. In order to solve this problem, we detected Mg by polymerase chain reaction(PCR) method. On the basis of the published nucleotide sequence of M. genitalium adhesion protein gene,two primers,MgPa-1 and MgPa-3,were synthesized. The results showed that amplification of Mg DNA yielded the predicted 281bp fragment, and amplification of M.pneumoniae, U.urealyticum,M.hominis,C.trachomatis and N.gonorrhoeae etc did not yield any fragment. It is the authors′, opinion that PCR is specific and sensitive, and it provides an important method for the laboratory diagnosis of Mg infection.The effects of primer concentration, annealing temperature and quantity of template on the result of amplification were discussed.

Key words: M.genitalium, Polymerase chain reaction