Abstract: A simple and rapid method of detection of ureapiasma urealyticum (UU) by Dat-immunobinding assay (DIBA) is described. It detected 10^3-4 ccu/ml of organisms. No cross-reaction appeared with the other 7 speties of mycoplasmas studied. Five hundred clinical specimens fmm gynecological patients wera tested for the presence of UU by the DIBA and culture method. The overall sensitivity of the DIBA was 93.7% with a specificity of 99.3%. Using chi-square method, P value <0.001. It indicated that the above two methods had a good agreement. The time requfired for detecting UU by culture was 7~9 days, whereas, the DIBA only 3~4h. For deteclion of UU, the DIBA is useful, easy and rapid as compared with the culture method.

Key words: Ureaplasma, urealyticum Dot-immunobinding assay