Abstract: According to ATPase Langerhans cell (LC) staining technique for light microscopy, we devised an improved ATPase technique which enables one to proceed from light to electron microscopic study. It made LC more easily found in electron mircoscopic observation by showing ATPase activity on ultrastructural level as well as its light micorscbpis count, and also provided an useful method for LC study under some pathologic conditions, especially when its antigens had changed. The method has the advantage of keeping intact most of the ATPase activity and ultrastructural details in comparison with others.