Abstract: 【Abstract】 Objective To compare and analyze the consistency of antifungal susceptibility of clinical isolates of Candida, Cryptococcus, and Aspergillus to 9 antifungal drugs between the Droplet48 method and the microbroth dilution method, as well as between the Droplet48 method and the Sensititre YeastOne method. Methods A total of 70 Candida strains, 40 Aspergillus strains, and 24 Cryptococcus strains isolated from clinical samples were tested for antifungal susceptibility. The minimum inhibitory concentrations （MICs） of fluconazole, voriconazole, itraconazole, posaconazole, amphotericin B, flucytosine, anidulafungin, micafungin, and caspofungin were determined using the microbroth dilution method, Sensititre YeastOne method, and Droplet48 method. The essential agreements of MIC values were evaluated between the microbroth dilution method and the Droplet48 method, as well as between the Sensititre YeastOne method and the Droplet48 method. Spearman's correlation analysis was carried out to evaluate the consistency between different methods by calculating the correlation coefficients of MIC values obtained by the Droplet48 method and the two reference methods. Results The essential agreements of MICs of the 9 antifungal drugs against Candida, Aspergillus, and Cryptococcus between the microbroth dilution method and the Droplet48 method were 84.3% - 92.9%, 85.0% - 92.5%, and 83.3% - 100%, respectively; the essential agreements between the Sensititre YeastOne method and the Droplet48 method for Candida, Aspergillus, and Cryptococcus were 85.7% - 94.3%, 90% - 95%, and 87.5% - 100%, respectively. The correlation coefficients of MIC values between the Droplet48 method and the reference methods ranged from 0.602 to 0.934. Spearman's correlation analysis showed that P values were all < 0.05, indicating good consistency of the Droplet48 method with the reference methods. Conclusion The Droplet48 method demonstrated good consistency with both the microbroth dilution method and the Sensititre YeastOne method in evaluating the antifungal susceptibility of Candida, Aspergillus, and Cryptococcus, proving to be a more automated and reliable approach.

Key words: Microbial sensitivity tests, Droplet48, Microbroth dilution method, Sensititre YeastOne