中华皮肤科杂志

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银屑病角质形成细胞p16启动子甲基化的研究

夏永华1, 李红文1, 丁一2   

  1. 1. 郑州大学第一附属医院皮肤科;
    2. 郑州大学医学院细胞分子生物学中心
  • 收稿日期:2005-09-09 出版日期:2006-03-15 发布日期:2006-03-15
  • 基金资助:
    河南省科技厅科研基金资助项目(20004025200)

The methylation of p16 promotor in psoriatic keratinocytes

XIA Yong-hua1, LI Hong-wen1, DING Yi2   

  1. Department of Dermatology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:2005-09-09 Online:2006-03-15 Published:2006-03-15

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摘要： 目的 探讨p16启动子甲基化与银屑病发生发展的关系.方法 采用甲基化特异性PCR及基因测序技术检测p16启动子.结果 26例银屑病患者皮损和非皮损甲基化率分别为23.08%(6/26)和19.23%(5/26),明显高于正常对照(P<0.05);12例进行期皮损和非皮损p16启动子甲基化率均为41.67%(5/12),14例静止期分别为7.14%(1/14)和0,进行期甲基化率明显高于静止期(P<0.05).结论 p16启动子甲基化与银屑病的发生发展相关,且与病期相关.

关键词: 银屑病, 基因,p16, 甲基化, 启动区(遗传学)

Abstract: Objective To study the relationship between the methylation of p16 promotor and the pathogenesis and progress of psoriasis. Method Methylation-specific PCR and DNA sequencing were used to detect the methylation of p16 promotor. Results The methylation of p16 promotor was found in 23.08% (6/26) of psoriatic lesions and 19.23% (5/26) of non-lesional areas in psoriatic patients, but none in normal controls. The frequency of methylation of p16 promotor was higher in psoriatic lesions than that in normal skin (P<0.05). The methylation frequencies of p16 promotor were 41.67% (5/12), 41.67% (5/12), respectively, in both psoriatic lesions and non-lesional areas in progressive phase, which were significantly higher in comparison with those in psoriatic lesions and non-lesional areas in stationary phase [7.14% (1/14) and 0(0/14), respectively] (P<0.05). Conclusion The methylation of p16 promotor may be related to the pathogenesis and progress of psoriasis.

Key words: Psoriasis, Genes,p16, Methylation, Promoter regions (genetics)