中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (5): 360-361.

• 研究报道 • 上一篇    下一篇

血清固定梅毒患者外周血CD4+CD25+Treg细胞IL-10的表达

张明海1,赵建斌2   

  1. 1. 安徽医科大学附属巢湖医院
    2. 徐州市第一人民医院
  • 收稿日期:2012-05-16 修回日期:2012-08-19 出版日期:2013-05-15 发布日期:2013-05-01
  • 通讯作者: 赵建斌 E-mail:zhaojianb@sina.com

Expression of interleukin 10 on peripheral blood CD+CD25+ regulatory T cells from serofast patients with syphilis

Zhang-Minghai ,Jian-Bin ZHAO   

  • Received:2012-05-16 Revised:2012-08-19 Online:2013-05-15 Published:2013-05-01
  • Contact: Jian-Bin ZHAO E-mail:zhaojianb@sina.com

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摘要： 目的 探讨CD4+CD25+调节性T细胞（CD4+CD25+Treg细胞）分泌的IL-10在梅毒血清固定现象形成中的作用。方法 流式细胞仪（FCM）检测28例血清固定梅毒患者和28例健康对照外周血CD4+CD25+IL-10+细胞比例及CD4+CD25+Treg细胞的IL-10分子数；同时用免疫磁珠细胞分选技术（MACS）分选出高纯度的CD4+CD25+Treg细胞后，再用实时PCR技术检测CD4+CD25+ Treg细胞的IL-10mRNA相对表达量。结果血清固定梅毒患者组外周血CD4+CD25+Treg细胞IL-10整体、蛋白和基因表达水平均比健康对照组明显增高，差异有统计学意义（均P < 0.01），但与快速血浆反应素环状卡片试验（RPR）比较，均无相关性（均P > 0.05）。结论 CD4+CD25+Treg细胞分泌的IL-10可能参与梅毒血清固定现象形成。

关键词: 梅毒, CD4+CD25+Treg细胞, 白细胞介素10

Abstract: ZHANG Ming-hai, ZHAO Jian-bin *. *Department of Dermatology, First People's Hospital of Xuzhou, Xuzhou 221002, Jiangsu, China Corresponding author: ZHAO Jian-bin, Email:zhaojianb@sina.com 【Abstract】 Objective To investigate the possible role of interleukin （IL）-10 secreted by peripheral CD+CD25+ regulatory T （Treg） cells in the formation of seroresistance state in patients with syphilis. Methods Venous blood samples were obtained from 28 serofast patients with syphilis and 28 healthy controls. Flow cytometry （FCM） was performed to determine the proportion of CD4+CD25+IL-10+ T cells in peripheral CD4+ cells, and to quantify the protein expression of IL-10 in CD4+CD25+ Treg cells. Magnetic activation cell sorting （MACS） system was used to separate CD4+CD25+ Treg cells in high purity followed by the detection of IL-10 mRNA expression with real time PCR. Results A significant increase was observed in the proportion of CD4+CD25+IL-10+ T cells in peripheral CD4+ cells, as well as protein and mRNA expression levels of IL-10 in the serofast patients with syphilis compared with the healthy controls （all P < 0.01）. However, no statistical correlation was observed between rapid plasma reagent （RPR） titer and any of the above three parameters in these patients （all P > 0.05）. Conclusion The high level of IL-10 secreted by CD4+CD25+ Treg cells may contribute to the formation of seroresistance state in patients with syphilis.

Key words: IL-10