中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (5): 362-364.

• 研究报道 • 上一篇    下一篇

颗粒溶素在不同类型药疹患者中的表达

潘月飞1,付萌2,高继鑫1,李巍3,乔洪江1,李春英4,李承新5,王刚6,高天文4,刘玉峰4   

  1. 1. 第四军医大学西京皮肤医院
    2. 西安第四军医大学西京医院全军皮肤性病中心
    3. 西安市第四军医大学西京医院皮肤科
    4. 西安第四军医大学西京医院皮肤科
    5. 第四军医大学西京医院全军皮肤病研究所
    6. 第四军医大学西京医院皮肤科
  • 收稿日期:2012-07-12 修回日期:2013-01-19 出版日期:2013-05-15 发布日期:2013-05-01
  • 通讯作者: 刘玉峰 E-mail:liuyuf@fmmu.edu.cn
  • 基金资助:
    陕西省科学技术研究发展计划项目

Expression of granulysin in patients with different types of drug eruption

  • Received:2012-07-12 Revised:2013-01-19 Online:2013-05-15 Published:2013-05-01

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摘要： 目的 比较不同类型药疹患者血清学及细胞中颗粒溶素表达差异。方法 酶联免疫吸附试验（ELISA）和流式细胞仪分别检测7例中毒性表皮坏死松解症、5例Stevens-Johnson综合征进展期患者、8例多形红斑药疹患者、8例发疹型药疹患者及11例健康人血清及外周血T细胞中颗粒溶素的表达水平。利用SPSS17.0进行统计学分析，两组均数比较采用随机或配对t检验，3组间比较采用ANOVA分析。结果 中毒性表皮坏死松解症和Stevens-Johnson综合征急性期患者血清颗粒溶素水平为（0.196 ± 0.079） μg/L，明显高于发疹型药疹患者［（0.022 ± 0.003） μg/L］和健康对照［（0.013 ± 0.005） μg/L］，F = 3.926，P < 0.05，但与多形红斑患者［（0.058 ± 0.004） μg/L］相比差异无统计学意义。Stevens-Johnson综合征患者和中毒性表皮坏死松解症患者表达颗粒溶素的CD8+ T细胞比例为（3.400 ± 0.754）%，多形红斑组为（0.600 ± 0.250）%，发疹型药疹组和健康对照组分别为（0.342 ± 0.251）%和（0.054 ± 0.024）%，4组间差异有统计学意义，F = 11.4，P < 0.01。结论 颗粒溶素在中毒性表皮坏死松解症、Stevens-Johnson综合征患者中表达水平较高，可能对该病急性期的鉴别诊断及预后评价具有潜在的应用价值。

关键词: 药疹, 颗粒溶素

Abstract: PAN Yue-fei, FU Meng, GAO Ji-xin, LI Wei, QIAO Hong-jiang, LI Chun-ying, LI Cheng-xin, WANG Gang, GAO Tian-wen, LIU Yu-feng. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Corresponding author: LIU Yu-feng, Email：liuyuf@fmmu.eud.cn 【Abstract】 Objective To compare the cellular and serum expression levels of granulysin among patients with different types of drug eruption. Methods Blood samples were collected from 7 patients with toxic epidermal necrolysis （TEN）, 5 patients with acute Stevens-Johnson syndrome （SJS）, 8 patients with erythema multiforme, 8 patients with exanthematic drug eruption, and 11 healthy controls. Flow cytometry and enzyme-linked immunosorbent assay （ELISA） were performed to quantify the protein expression of granulysin in peripheral blood T cells and sera from these subjects, respectively. Data were processed by the SPSS17.0 software, and differences in these parameters were analyzed by one-way analysis of variance and independent or paired sample t-test. Results The serum level of granulysin in patients with TEN or acute SJS was significantly higher than that in those with exanthematic drug eruption and healthy controls （（0.196 ± 0.079） μg/L vs.（0.022 ± 0.003） μg/L and （0.013 ± 0.005） μg/L, F = 3.926, P < 0.05）, but similar to that in those with erythema multiforme （（0.058 ± 0.004 ） μg/L, P > 0.05）. Significant differences were observed in the proportion of CD8+ T cells expressing granulysin in peripheral blood mononuclear cells （PBMCs） between patients with TEN or acute SJS, erythema multiforme, exanthematic drug eruption, and healthy controls （（3.400 ± 0.754）% vs. （0.600 ± 0.250）% vs. （0.342 ± 0.251）% vs. （0.054 ± 0.024）%, F = 11.4, P < 0.01）. Conclusions Granulysin is highly expressed in patients with TEN and SJS, which may have potential value for their differential diagnosis and prognosis evaluation at acute phase.

Key words: Granulysin