中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 13-15.

• 论著 • 上一篇    下一篇

特应性皮炎患儿血清和CD4+CD25+ T细胞分泌IL-10水平的检测

金宛宛1,胡波瑛2,马新华3,陈仕胜4,高宇4   

  1. 1. 温州医科大学附属第二医院皮肤科
    2. 宁波市江东区妇幼保健院
    3.
    4. 温州医学院附属第二医院皮肤科
  • 收稿日期:2012-07-17 修回日期:2012-09-13 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 高宇 E-mail:gaoyu0570@163.com
  • 基金资助:
    浙江省温州市科技局对外合作课题

Detection of interleukin-10 in sera and culture supernatants of CD4+CD25+ T cells from children with atopic dermatitis

  • Received:2012-07-17 Revised:2012-09-13 Online:2013-01-15 Published:2013-01-01

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摘要： 目的 探讨儿童特应性皮炎血清和CD4+CD25+T分泌细胞因子IL-10表达，分析其与病程及严重程度的相关性。 方法 特应性皮炎患儿按SCORAD指数分3组，轻度10例、中度16例、重度20例。抽取46例特应性皮炎患儿和31例健康对照儿外周血，用免疫磁珠分离获得CD4+CD25+T细胞，用ELISA法分别检测患病组和健康对照组血清及CD4+CD25+T细胞培养液IL-10水平，并分析IL-10水平与SCORAD评分的相关性。结果 轻、中、重度AD各组血清IL-10水平分别为（43.10 ± 25.07）、（68.40 ± 36.65）、（55.55 ± 41.97） pg/ml，与健康对照组（58.27 ± 36.84） pg/ml比较，差异均无统计学意义（P ＞ 0．05），与患儿SCORAD评分无相关性。轻、中、重度AD组CD4+CD25+T细胞分泌IL-10含量分别为（52.96 ± 11.69）、（49.86 ± 9.18）、（27.25 ± 7.01） pg/ml，重度AD组低于健康对照组（55.15 ± 11.15） pg/ml （P < 0．05）；轻、中度组与健康对照组差异无统计学意义（P ＞ 0．05）。CD4+CD25+T细胞分泌IL-10的水平与患儿疾病严重程度SCORAD评分呈明显负相关（r值分别为-0．757，P < 0．01）。结论 CD4+CD25+T细胞及相关因子IL-10可能参与儿童特应性皮炎的发病。

关键词: 皮炎，特应性, CD4淋巴细胞计数, 白细胞介素10

Abstract: JIN Wan-wan*, HU Bo-ying, MA Xin-hua, CHEN Shi-sheng, GAO Yu. *Department of Dermatology, Second Affiliated Hospital and Yuying Children′s Hospital of Wenzhou Medical College, Wenzhou 325027, Zhejiang, China Corresponding author: GAO Yu, Email: gaoyu@medmail.com.cn 【Abstract】 Objective To determine the level of interleukin （IL）-10 in sera and culture supernatants of CD4+CD25+ T cells from children with atopic dermatitis（AD）, and to evaluate its relationship with clinical course and severity of AD. Methods Totally, 46 children with AD and 31 healthy controls were included in the study. All the patients were divided into 3 groups, i.e., mild （n = 10）, moderate （n = 16） and severe （n = 20） group, according to severity scoring of atopic dermatitis （SCORAD） score. Venous blood samples were obtained from the patients and healthy controls. CD4+CD25+ regulatory T cells were separated from the blood samples by magnetic cell sorting （MACS） system in two steps and cultured in vitro． Enzyme linked immunosorbent assay （ELISA） was conducted to quantify the level of IL-10 in sera and culture supernatants of CD4+CD25+ T cells from these subjects. Analysis of variance was carried out to compare the level of supernatant and serum IL-10 between the patients and controls, and Pearson correlation analysis to assess the relationship between the level of IL-10 and SCORAD score. Results The patients with mild, moderate and severe AD showed a similar serum IL-10 level compared with the healthy controls （（43.10 ± 25.07） pg/ml, （68.40 ± 36.65） pg/ml and （55.55 ± 41.97） pg/ml vs. （58.27 ± 36.84） pg/ml, all P > 0.05）. The level of supernatant IL-10 secreted by CD4+CD25+ T cells from the controls was significantly higher than that from the patients with severe AD （（55.15 ± 11.15） pg/ml vs. （27.25 ± 7.01） pg/ml, P < 0.05）, but similar to that from the patients with mild and moderate AD （（52.96 ± 11.69） pg/ml and （49.86 ± 9.18） pg/ml, respectively, both P > 0.05） . The level of secreted IL-10 was negatively correlated with SCORAD score（r = －0.757, P < 0.01）, whereas the serum level of IL-10 showed no statistical correlation with SCORAD score. Conclusion CD4+CD25+ T cells and IL-10 may be implicated in the development of AD. 【Key words】 Dermatitis, atopic; CD4 lymphocyte count; Interleukin-10

Key words: cytokines-10