中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 9-12.

• 论著 • 上一篇    下一篇

CD200在斑秃患者淋巴细胞和毛囊上的表达和意义

管海宏1,2,李慧2,李泓馨3,林麟4,王洪生4,周武庆5,5   

  1. 1. 南京军区南京总医院皮肤科
    2. 中国医学科学院皮肤病研究所
    3. 中国医学科学院皮肤病研究所皮研室
    4. 南京 中国医学科学院北京协和医学院皮肤病研究所
    5. 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2012-06-18 修回日期:2012-07-25 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 林麟 E-mail:dllon3@163.com

Expression of CD200 in peripheral lymphocytes and hair follicles from patients with alopecia areata and its significance

  • Received:2012-06-18 Revised:2012-07-25 Online:2013-01-15 Published:2013-01-01

PDF

37

赞

摘要： 目的 探讨CD200在斑秃患者外周血淋巴细胞和头皮毛囊上的表达。方法 用流式细胞仪检测CD200在43例斑秃患者外周血淋巴细胞上的表达。免疫组化法检测CD200和正常毛囊外根鞘基底层标记CK15（cytokeratin 15）在8例斑秃患者毛囊上的表达，以同等例数健康人的标本做对照，进行分析比较。 结果 CD200在斑秃患者外周血总淋巴细胞上及T淋巴细胞上的表达率分别为5.73% ± 3.46%，8.85% ± 4.8%，明显低于健康对照人群（12.01% ± 4.9%；12.31% ± 3.12%，t值分别为6.865，3.964，P值均 ＜ 0.05；而在患者B淋巴细胞上的表达比例为74.68 ± 8.12，较健康对照人群差异无统计学意义（75.750 ± 9.447， t = 0.570，P > 0.05）；斑秃患者毛囊上CD200的表达较健康对照人群亦明显下降（P ＜ 0.05），而CK15较健康对照无明显下降。结论 CD200在斑秃患者外周血淋巴细胞和毛囊上表达的下降可能参与了斑秃的发病。

关键词: 斑秃, CD200, 毛囊, 淋巴细胞

Abstract: GUAN Hai-hong, LI Hui, LI Hong-xin, LIN Lin*, WANG Hong-sheng, ZHOU Wu-qing. *Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China Corresponding author: LIN Lin, Email:dllon3@yahoo.com.cn 【Abstract】 Objective To investigate the expression of CD200 in peripheral lymphocytes and hair follicles from patients with alopecia areata （AA）. Methods Flow cytometry was used to detect the expression of CD200 in peripheral lymphocytes from 43 patients with AA and 43 healthy controls. Immunohistochemistry was carried out to quantify the expression of CD200 and cytokeratin 15（CK15, a marker for basal cells of the outer root sheath） in resected scalp specimens from 8 patients with AA and 8 healthy controls. Differences in the expression of CD200 and CK15 between the patients and controls were assessed by independent-samples t test and rank sum test. Data were processed by the software SPSS17.0. Results The percentage of CD200-expressing cells in peripheral blood lymphocytes and T lymphocytes was significantly lower in the patients with AA than in the healthy controls （5.73% ± 3.46% vs. 12.01% ± 4.9%, 8.85% ± 4.8% vs. 12.31% ± 3.12%, t = 6.865, 3.964, respectively, both P < 0.05）. However, no significant difference was observed in the percentage of CD200-expressing cells in peripheral blood B lymphocytes between the patients and controls （74.676% ± 8.122% vs. 75.750% ± 9.447%, t = 0.570, P > 0.05）. Further more, the patients showed a lower expression of CD200 （P < 0.05）, but a similar expression of CK15 （P > 0.05） in hair follicles compared with the controls. Conclusion The decrease in CD200 expression in peripheral lymphocytes and hair follicles may be involved in the pathogenesis of AA. 【Key words】 Alopecia areata; CD200; Lymphocytes; Hair follicle

Key words: alopecia areata, CD200