中华皮肤科杂志 ›› 2012, Vol. 45 ›› Issue (12): 862-864.

• 论著 • 上一篇    下一篇

扁平苔藓皮损中肿瘤坏死因子α和半胱氨酸天冬氨酸蛋白酶8、3的表达

王娟1,白莉2,鲍海平1,郑爱义1,武晓华1,赵一锦1,罗燕米希婷1   

  1. 1. 山西大同大学医学院
    2. 山西省太原市山西医科大学第一医院皮肤科
  • 收稿日期:2012-02-01 修回日期:2012-04-05 出版日期:2012-12-15 发布日期:2012-11-30
  • 通讯作者: 王娟 E-mail:wangjuan2884762@126.com

Expressions of tumor necrosis factor-α, caspase-8 and caspase-3 in lichen planus lesions

  • Received:2012-02-01 Revised:2012-04-05 Online:2012-12-15 Published:2012-11-30

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摘要：

目的 研究扁平苔藓皮损中肿瘤坏死因子α（TNF-α）、半胱氨酸天冬氨酸蛋白酶8（Caspase-8）和Caspase-3的表达及意义。方法 采用免疫组化法和原位末端标记技术（TUNEL）检测20例扁平苔藓患者皮损和20例健康人皮肤组织中TNF-α、Caspase-8、Caspase-3的表达和细胞凋亡情况。采用SPSS11.5统计软件，组间比较采用成组t检验，指标间相关性采用Pearson相关分析。结果 扁平苔藓组TNF-α、Caspase-8和Caspase-3的积分光密度值分别为12580 ± 2330、11690 ± 3520和11450 ± 2820，均明显高于健康对照组（分别为5120 ± 1780、3870 ± 3360、4760 ± 1930），两组比较，t值分别为11.38、7.19、8.76，P值均 ＜ 0.01；扁平苔藓组角质形成细胞凋亡指数（71.35 ± 7.93），明显高于健康对照组（33.62 ± 8.75），两组比较，t = 14.29，P ＜ 0.01。扁平苔藓组TNF-α、Caspase-8的表达强度与角质形成细胞凋亡指数间均呈正相关，r分别为0.72、0.75，P值均 ＜ 0.01；同时两者与Caspase-3的阳性表达也均呈正相关，r分别为0.68、0.73，P值均 ＜ 0.01。结论 TNF-α、Caspase-8 和Caspase-3的高表达可能参与了扁平苔藓中角质形成细胞的凋亡。

关键词: 细胞凋亡

Abstract:

Objective To quantify the expressions of tumor necrosis factor-α （TNF-α）, caspase-8 and caspase-3 in lichen planus （LP） lesions, and to investigate their significance. Methods Skin samples were collected from the lesions of 20 patients with LP and normal skin of 20 healthy human controls. Immunohistochemistry was used to determine the expressions of TNF-α, caspase-8 and caspase-3, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling （TUNEL） technique to evaluate the apoptosis in keratinocytes, in these samples. Results The expression levels（expressed in integrated optical density, IOD） of TNF-α, caspase-8 and caspase-3 were （12.58 ± 2.33） × 103, （11.69 ± 3.52） × 103 and （11.45 ± 2.82） × 103 respectively in LP lesions, significantly higher than those in the normal skin （（5.12 ± 1.78） × 103, （3.87 ± 3.36） × 103, （4.76 ± 1.93） × 103, t = 11.38, 7.19, 8.76，respectively, all P ＜ 0.01）. Elevated apoptosis index was noted in keratinocytes from LP lesions compared with those from normal skin （71.35 ± 7.93 vs. 33.62 ± 8.75, t = 14.29, P < 0.01）. In LP lesions, the expressions of both TNF-α and caspase-8 were positively correlated with the apoptosis index of keratinocytes （r = 0.72, 0.75, respectively, both P < 0.01） and the expression of caspase-3 （r = 0.68, 0.73, respectively, both P ＜ 0.01）. Conclusion The up-regulated expressions of TNF-α, caspase-8 and caspase-3 may participate in the apoptosis in keratinocytes in LP.

Key words: Apoptosis