中华皮肤科杂志 ›› 1994, Vol. 27 ›› Issue (5): 284-285,329.

• 论著 • 上一篇    下一篇

银屑病皮损中γ干扰素的免疫组化分析

赵庆利1, 刘玮1, 关鹏举2, 王伯云2, 肖曼西2   

  1. 1. 中国人民解放军空军总医院皮肤科, 北京100036;
    2. 第四军医大学唐都医院皮肤科
  • 收稿日期:1993-10-28 修回日期:1994-01-26 出版日期:1994-10-15 发布日期:1994-10-15

Immunohistochemical Studies of γ-Interferon in Psoriatic Lesions

ZHAO Qing-Li1, LIU Wei1, GUAN Peng-Jü2   

  1. Department of Dermatology, General Hospital of Air Force, PLA, Beijing 100036
  • Received:1993-10-28 Revised:1994-01-26 Online:1994-10-15 Published:1994-10-15

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摘要： 采用γ干扰素(IFN-γ)单克隆抗体、双层APAAP染色法及显微分光光度测定技术对30例银屑病患者皮损、15例正常对照皮肤进行IFN-γ抗原的测定。结果显示:除表皮基底层及表皮突下部1～3层基层外,大量的IFN-γ抗原阳性染色弥漫分布于皮损全层表皮的角肮细胞间隙;正常对照皮肤的表皮中无明确的IFN-γ阳性反应;银屑病皮损表皮中IFN-γ的含量①与银屑病的活动性有关,进行期为24.3808.250,静止期为15.955±5.327(P<0.01);②与皮损表皮中T细胞、HLA-DR细胞的数量呈直线正相关(r1=0,694,r2=0.436,P<0.05).

关键词: 干扰素类Ⅱ型, 银屑病

Abstract: The skin biopsies from thirty patients with psoriasis and fifteen normal controls were examined for-interferon (IFN-γ) by using murine monoclonal antibodies against human IFN-γ, immunohistochemical technique (APAAP method) and microspectrophotometry. Our results showed that the staining for IFN-γ could be detected in the epidermis as well as in the dermis of all the psoriatic lesions, with more intense staining and wider distribution in lesional epidermis than in lesional dermis. IFN-γ was observed in the intercellular spaces of the keratinocytes in the upper and mid layers of epidermis, but not in stratum basale and first 1-3 cell rows of stratum spinosum. The IFN-γ levels were increased in the lesional epidermis of active stage and decreased in the stationary stage. No positive staining was found in the skin of the normal controls.

Key words: Interferon, type Ⅱ, Psoriasis