中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (1): 22-24.

• 论著 • 上一篇    下一篇

丹参对系统性硬皮病患者皮肤成纤维细胞增殖和胶原合成的影响

李明1, 王强1, 杨春欣2, 陈蕾1, 秦万章1   

  1. 1. 上海医科大学中山医院皮肤科, 200032;
    2. 上海医科大学中山医院中草药制剂室
  • 收稿日期:1997-03-27 修回日期:1997-10-10 出版日期:1998-02-15 发布日期:1998-02-15
  • 基金资助:
    卫生部科研基金

Effects of Salvia Miltiorrhiza Bunge on the Proliferation and Collagen Synthesis in Systemic Scleroderma Skin Fibroblasts

Li Ming1, Wang Qiang1, Yang Chunxin2   

  1. Department of Dermatology, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032
  • Received:1997-03-27 Revised:1997-10-10 Online:1998-02-15 Published:1998-02-15

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摘要： 目的 探讨丹参治疗系统性硬皮病的药理机制。方法 用四甲基偶氮唑盐 (MTT)方法和光度检测法测定了丹参对成纤维细胞增殖和培养基中胶原含量的影响。结果 丹参水溶性提取物丹参注射液 (10 mg/ml、7.5 mg/ml、 5.0 mg/ml、2.5 mg/ml)及其活性单体丹参素 (60 μg/ml、 45 μg/ml、 30 μg/ml、15 μg/ml)和原儿茶醛 (40 μg/ml、20 μg/ml、10 μg/ml、5 μg/ml),以及丹参脂溶性总提取物 (1mg/ml、0.5 mg/ml、0.25 mg/ml)及其活性单体丹参酮 A (4μg/ml,2μg/ml),不仅对患者皮肤成纤维细胞的增殖具有显著的抑制作用,还能显著降低该细胞培养基中可溶性胶原的含量 (P<0.05～0.01)。上述抑制作用随药物浓度的增高或作用时间的延长而增强。采用流式细胞仪对该细胞周期的检测显示,丹参素组G0/G1期细胞,原儿茶醛组和丹参酮A组G2+ M期细胞的比例较正常人对照组细胞显著增高 (P<0.01)。结论 丹参对体外培养的系统性硬皮病患者的皮肤成纤维细胞的增殖和胶原合成具有显著的抑制作用,可能是其治疗系统性硬皮病的药理机理之一。

关键词: 丹参, 硬皮病,系统性, 成纤维细胞

Abstract: Objective To study the pharmacologic mechanism of Salvia miltiorrhiza Bunge(SMB) in the treatment of systemic scleroderma(SSc). Methods We used the cultured SSc skin fibroblasts as an experimental model and detected the effects of SMB both on the cell proliferation by MTT assay and on the collagen production in the culture medium by photometric determination.Results The results showed that the SMB water soluble extracts,such as SMB injection (10 mg/ml、7.5 mg/ml、 5 mg/ml、2.5 mg/ml) and its effective monomer Danshensu (60 μg/ml、45 μg/ml、30 μg/ml、15 μg/ml) and protocate chualdehyde (40μg/ml、 2 0μg/ml、 10μg/ml、 5μg/ml),as well as SMB total liposoluble extract (1mg/ml、0.5 mg/ml、0.25 mg/ml) and its effective monomer tanshinone A(4μg/ml、2 μg/ml),could not only significantly inhibit the proliferation of the cultured SSc skin fibroblasts,but also significantly reduce the soluble collagen production in the culture medium(P<0.05～0. 0 1).The inhibiting effect was increased along with the increasing the concentration of the drugs or the prolonging the time of action. The cell cycle determination by flow cytometer(FCM) analysis showed that the cell proportion in G0/G1 phases after treated by Danshensu and in G2+ M phases by protocate chualdehyde and tanshinone A increased significantly than the controls(P<0.01).Conclusion The above results demonstrated that SMB could significantly inhibit the proliferation and the collagen synthesis in cultured SSc skin fibroblasts and this maybe one of its pharmacologic mechanismin the treatment of SSc.

Key words: Scleroderma, systemic, Fibroblast, Salvia miltiorrhiza