中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (4): 233-235.

• 论著 • 上一篇    下一篇

表皮角朊细胞和真皮成纤维细胞血小板衍生生长因子的产生及调节

张建中1, 郑学毅2, 马圣清2, 金子史男3   

  1. 1. 北京医科大学人民医院皮肤科 100044;
    2. 北京医科大学第一医院皮肤科;
    3. 日本福岛医科大学皮肤科
  • 收稿日期:1997-06-02 修回日期:1998-01-19 出版日期:1998-08-15 发布日期:1998-08-15

Production and Regulation of Platelet-Derived Growth Factor in Keratinocytes and Dermal Fibroblasts

Zhang Jiangzhong1, Zhen Xueyi2, Ma Shengqing2   

  1. Department of Dermatology, People's Hospital, Beijing Medical University, Beijing 100044
  • Received:1997-06-02 Revised:1998-01-19 Online:1998-08-15 Published:1998-08-15

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摘要： 目的 探讨血小板衍生生长因子(PDGF)在表皮角朊细胞和真皮成纤维细胞的产生及其调节因素.方法 培养人角朊细胞和成纤维细胞,用ELISA法测定PDGF多肽分子,用Northern杂交检测PDGF-B链mRNA的表达.结果 人角朊细胞可合成PDGF多肽分子,双羟维生素D3可促进其合成,肉豆寇沸波酯(PMA)可抑制其合成.成纤维细胞不产生PDGF分子,可测到低水平的PDGFB链mRNA表达.结论 表皮角朊细胞是皮肤中PDGF分子的一个重要产生细胞,成纤维细胞不能产生PDGF可能是由于存在翻译阻抑因素.

关键词: 角蛋白细胞, 成纤维细胞, 血小板源生长因子

Abstract: Objective To evaluate the production and regulation of platelet-derived growth factor (PDGF) in the skin. Methods Normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs) were cultured, PDGF peptides were measured by ELISA and PDGF-B chain mRNA was detected by Northern hybridization. Results The results showed that NHKs constitutively produced PDGF molecules, phorbol 12-myristate 13-acetate (PMA) inhibited PDGF production, while 1, 25-dihydroxyvitamin D3 enhanced its production. TNF-alpha, interferon-γ and interleukin-1 alpha all failed to affect PDGF production. On the other hand, HDFs produced no PDGF molecule in spite of a low level of PDGF-B chain mRNA expression in Northern hybridization, suggestting a post-transcriptional blocking of PDGF production in HDFs. Conclusion The results obtained above indicate that epidermal keratinocytes may be the major PDGF-generating cells, whereas dermal fibroblasts the PDGF-responsive cells in the skin.

Key words: Keratinocytes, Fibroblasts, Platelet-derived growth factor