中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (4): 227-229.

• 论著 • 上一篇    下一篇

整合素表达对硬皮病成纤维细胞合成原胶原的影响

洪微, 廖万清, 孔宪涛, 陈敏亮, 李志刚, 何金   

  1. 1. 第二军医大学附属长征医院皮肤科 上海 200003;
    2. 第二军医大学附属长征医院实验科;
    3. 第二军医大学附属长征医院整形外科;
    4. 第二军医大学附属长征医院病理科
  • 收稿日期:1997-08-10 修回日期:1997-11-05 出版日期:1998-08-15 发布日期:1998-08-15
  • 基金资助:
    国家自然科学基金资助项目

Effect of Integrin Expression on Procollagen Synthesis of Fibroblasts from Scleroderma

Hong Wei, Liao Wanqin, Kong Xiantao   

  1. Department of Dermatology, Changzheng Hospital, the Secend Military Medical University, Shanghai 200003
  • Received:1997-08-10 Revised:1997-11-05 Online:1998-08-15 Published:1998-08-15

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摘要： 目的 研究硬皮病成纤维细胞整合素表达对成纤维细胞合成原胶原的影响.方法 运用硫代修饰的整合素反义寡核苷酸阻断硬皮病成纤维细胞表面相应整合素亚基的表达,RT-PCR检测整合素表达抑制后硬皮病成纤维细胞原胶原mRNA量的变化.结果 反义寡核苷酸能特异抑制硬皮病成纤维细胞相应整合素亚基的表达;整合素α5或β1亚基表达减少的成纤维细胞,原胶原proα1(Ⅰ)、proα1(Ⅲ)mRNA均显着降低.结论 降低硬皮病成纤维细胞整合素的表达,可从转录水平抑制成纤维细胞合成原胶原.

关键词: 硬皮病,系统性, 硬皮病,局限性, 成纤维细胞, 结合素类, 原胶原

Abstract: Objective Investigation of the effect of integrin on fibroblasts from scleroderma in the production of procollagen. Methods Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin α5 or β1 subunit on scleroderma fibroblasts respectively, then the changes of procollagen mRNA were detected by RT-PCR. Results The expression of integrin α5 or β1 subunit could be specifically inhibited by their antisense oligonucleotides respectively. Decreased expression of fibroblast integrin α5 or β1 subunit significantly lowered mRNA of procollagen α1(Ⅰ) and α5(Ⅲ). Conclusion Overproduction of procollagen may be inhibited in the level of transcription by lowering the expression of integrin on fibroblasts in scleroderma.

Key words: Scleroderma,systemic, Scleroderma,circumscribed, Fibroblasts, Integrins, Procollagen