中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 572-574.

• 论著 • 上一篇    下一篇

抗角蛋白自身抗体对角质形成细胞凋亡的影响

孙林潮, 赵小东, 刘玉峰, 樊平申, 李承新, 卢涛, 李巍, 高天文   

  1. 第四军医大学西京医院皮肤科, 西安710032
  • 收稿日期:2004-09-27 发布日期:2005-09-15
  • 基金资助:
    国家自然科学基金资助重点项目(30330510)

Effects of Anti-Keratin Autoantibody on Apoptosis of Cultured Human Keratinocytes

SUN Lin-chao, ZHAO Xiao-dong, LIU Yu-feng, FAN Ping-shen, LI Cheng-xin, LU Tao, LI Wei, GAO Tian-wen   

  1. Department of Dermatology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China
  • Received:2004-09-27 Published:2005-09-15

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摘要： 目的 探讨抗角蛋白自身抗体(AK auto Ab)对角质形成细胞凋亡的影响。方法 以不同浓度AK auto Ab作用体外培养人角质形成细胞后,用光镜及透射电镜观察细胞形态变化、用流式细胞仪分析细胞周期变化以及提取角质形成细胞DNA作电泳特征分析。结果 AK auto Ab作用后培养的角质形成细胞光镜、电镜下形态发生凋亡特有的改变,出现核固缩、染色质凝聚,并形成凋亡小体;细胞周期分析图中出现凋亡峰;DNA电泳呈有一定间隔的梯状条带。结论 AK auto Ab对角质形成细胞的凋亡具有诱导作用。

关键词: 自身抗体, 角蛋白, 角蛋白细胞, 细胞凋亡

Abstract: Objective To study the effects of anti-keratin autoantibody (AK auto Ab) on apoptosis of human keratinocytes. Methods Light and electron microscopy were used to observe morphological changes, flow cytometry (FCM) to analyze cell cycle, and electrophoresis to analyze DNA profiles of cultured keratinocytes. Results Typical morphological changes with apoptotic characteristics such as karyopyknosis, chromatin agglutination and apoptosis bodies were found. The subdiploid peaks due to apoptosis were also found in cell cycle analyses. DNA electrophoresis of keratinocytes showed characteristic ladder. Conclusions AK auto Ab could induce apoptosis in cultured human keratinocytes.

Key words: Autoantibodies, Keratin, Keratinocytes, Apoptosis