中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 543-545.

• 论著 • 上一篇    下一篇

人乳头瘤病毒cp6108的序列研究

蒋明军, 王书崎, 余艳华, 尹跃平, 韩国柱, 孙建方   

  1. 中国医学科学院中国协和医科大学皮肤病研究所, 210042
  • 收稿日期:2005-01-26 发布日期:2005-09-15

DNA Sequencing of HPV cp6108 Gene

JIANG Ming-jun, WANG Shu-qi, YU Yan-hua, YIN Yue-ping, HAN Guo-zhu, SUN Jian-fang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2005-01-26 Published:2005-09-15

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摘要： 目的 对尖锐湿疣中人乳头瘤病毒(HPV)cp6108进行序列研究。方法 应用TA克隆后测序和PCR产物直接测序进行HPV cp6108的基因序列测定。结果 5例HPV cp6108阳性的尖锐湿疣的模板DNA经TA克隆后测序和PCR产物直接测序结果显示,两种测序结果一致,BLAST比对结果显示,与基因库中HPV cp6108提交序列有99%的一致性,排在比对结果第1位,所有标本的cp6108的序列与网上提交序列对比后发现均有3处相同的密码子发生改变(以起始密码子GCA之G为第1位):第210位核苷酸G(鸟嘌呤,)突变为A(腺嘌呤),导致第70位密码子GAG→GAA,发生同义突变;第229位核苷酸G(鸟嘌呤)突变为A(腺嘌呤),导致第77位密码子GAT→AAT,编码的氨基酸由天冬氨酸(Asp,D)变为天冬酰氨(Asn,N),即发生D77N错义突变;第346位核苷酸A(腺嘌呤)突变为C(胞嘧啶),导致第116位ACT→CCT,编码的氨基酸由苏氨酸(Thr,T)变为脯氨酸(Pro,P),即发生T116P错义突变。结论 与基因库提交序列相比,本研究检测到的HPV cp6108序列中有3处突变,其中G70A为同义突变,D77N和T116P为错义突变。

关键词: 乳头状瘤病毒, 人, HPV cp6108, 序列分析, DNA

Abstract: Objective To study LI gene sequence of HPV cp6108 from 5 cases of condyloma acuminata. Methods T-A cloning and direct sequencing of PCR product were used. Results The LI gene sequences of HPV cp6108 from 5 specimens were presented with the homology of 99% to reference sequence in GenBank. A total of 3 gene mutations were found, including a nonsense mutation of G70A, a missense mutation of D77N, and a missense mutation of Tl16P. Conclusions In comparison with the sequence in GenBank, at least 3 gene mutations of HPV CP6108, i.e. one nonsense mutation of G70A and missense mutations of D77N and Tl 16P, are found in the present study.

Key words: Papillomavirus, human, HPV cp6108, Sequence analysis, DNA