银屑病患者外周血单核细胞向树突状细胞分化能力的研究

中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (2): 88-90.

银屑病患者外周血单核细胞向树突状细胞分化能力的研究

朱可建, 周伟芳, 劳力民, 郑敏

浙江大学医学院附属第二医院皮肤科杭州 310009

收稿日期:2001-10-24 出版日期:2002-04-15 发布日期:2002-04-15

Differentiation of Monocyte derived Dendritic Cells in Patients with Psoriasis

ZHU Kejian, ZHOU Weifang, LAO Limin, ZHENG Min

Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China

Received:2001-10-24 Online:2002-04-15 Published:2002-04-15

摘要/Abstract

摘要： 目的了解银屑病患者外周血单核细胞向树突状细胞(DC)分化的能力。方法采用流式细胞仪检测细胞表面抗原表达,同种混合淋巴细胞反应评价细胞的抗原递呈能力。结果银屑病患者外周血单核细胞能向DC分化,分化而成的DC表达CD40、CD80、CD86和HLA-DR的阳性率显着高于正常人(P<0.01),刺激淋巴细胞增殖的能力显着强于正常人(P<0.01)。结论银屑病患者外周血单核细胞向DC分化的能力增强,分化而成的DC具有很强的抗原递呈能力。

关键词: 银屑病, 树突细胞, 细胞分化, 单核细胞

Abstract: Objective To investigate the differentiation of dendritic cells from monocytes of the peripheral blood in patients with psoriasis. Methods Flow cytometry was used to analyze the phenotype of monocyte derived dendritic cells (MoDC). The capacity of MoDC to stimulate the proliferation of T lymphocytes was evaluated by allogeneic mixed lymphocyte reaction. Results Monocytes in the peripheral blood of patients with psoriasis could differentiate into dendritic cells. Expression of CD40, CD80, CD86 and HLA-DR by MoDC was significantly increased in patients with psoriasis compared with that in normal controls (P<0.01). The capacity of MoDC to stimulate T lymphocytes was enhanced significantly in patients'group as well (P<0.01). Conclusions The differentiation of monocytes into dendritic cells is upregulated in psoriasis, and these cells have potent antigen presenting capacities.

Key words: Psoriasis, Dendritic cells, Cell differentiation, Monocytes

朱可建, 周伟芳, 劳力民, 郑敏. 银屑病患者外周血单核细胞向树突状细胞分化能力的研究[J]. 中华皮肤科杂志, 2002, 35(2): 88-90.

ZHU Kejian, ZHOU Weifang, LAO Limin, ZHENG Min. Differentiation of Monocyte derived Dendritic Cells in Patients with Psoriasis[J]. Chinese Journal of Dermatology, 2002, 35(2): 88-90.

参考文献

[1] Nestle FO, Turka LA, Nickoloff BJ.Characterization of dermal dendritic cells in psoriasis.Autostimulation of T lymphocytes and induction of Th1 type cytokines.J Clin Invest, 1994,94:202-209.
[2] Dam TN, Moller B, Hindkjaer J, et al.The vitamin D3 analog calcipotriol suppresses the number and antigen-presenting function of Langerhans cells in normal human skin.J Investig Dermatol Symp Proc, 1996,1:72-77.
[3] Zhu K, Mrowietz U.Inhibition of dendritic cell differentiation by fumaric acid esters.J Invest Dermatol, 2001,116:203-208.
[4] Cooper KD, Baadsgaard O, Ellis CN, et al.Mechanisms of cyclosporin A inhibition of antigen-presenting activity in uninvolved and lesional psoriatic epidermis.J Invest Dermatol, 1990, 94:649-656.
[5] Sallusto F, Lanzavecchia A.Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.J Exp Med, 1994,179:1109-1118.
[6] Valdimarsson H, Sigmundsdottir H, Jonsdottir I.Is psoriasis induced by streptococcal superantigens and maintained by M-protein-specific T cells that cross-react with keratin? Clin Exp Immunol, 1997,107(Suppl 1):21-24.
[7] Drakesmith H, Chain B, Beverley P.How can dendritic cells cause autoimmune disease? Immunol Today, 2000,21:214-217.
[8] Geissmann F, Prost C, Monnet JP, et al.Transforming growth factor beta 1, in the presence of granulocyte/macrophage colony-stimulating factor and interleukin 4, induces differentiation of human peripheral blood monocytes into dendritic Langerhans cells.J Exp Med, 1998,187:961-966.
[9] Asadullah K, Sterry W, Stephanek K, et al.IL-10 is a key cytokine in psoriasis.Proof of principle by IL-10 therapy:a new therapeutic approach.J Clin Invest, 1998,101:783-794.

[1]	刘娜覃慧郑捷. 组织常驻记忆性T细胞与银屑病[J]. 中华皮肤科杂志, 2018, 51(8): 630-632.
[2]	毛旭华栾超胡煜蒋明军陈敏. 寻常性银屑病患者表皮中DNA甲基化转移酶2和3a mRNA的表达[J]. 中华皮肤科杂志, 2018, 51(8): 607-608.
[3]	张瑞丽朱小红华海康杨莉佳. 阿达木单抗治疗重度银屑病14例临床疗效及安全性观察[J]. 中华皮肤科杂志, 2018, 51(8): 604-606.
[4]	周文娇任杰韩珑刘欣欣汤坤龙罗素菊. 白细胞介素22对人表皮角质形成细胞CC趋化因子配体27表达的影响[J]. 中华皮肤科杂志, 2018, 51(8): 592-594.
[5]	郝阳阳张梁宇王翔童云峰孙颖郑丽君陈杨. 喜树碱对人原代角质形成细胞自噬的影响[J]. 中华皮肤科杂志, 2018, 51(7): 510-514.
[6]	段志敏杜蕾蕾刘彩霞曾荣沈永年胡素泉刘维达陈青李岷. Dectin-1介导人急性单核细胞白血病细胞巨噬细胞样细胞吞噬白念珠菌的作用研究[J]. 中华皮肤科杂志, 2018, 51(6): 425-428.
[7]	朱腾晋红中. 泛发性脓疱性银屑病患者外周血单个核细胞中程序性细胞死亡受体1及其下游因子相关表达研究[J]. 中华皮肤科杂志, 2018, 51(6): 451-453.
[8]	吕静张颖闫国富刘娟娟. 重症银屑病并发双眼葡萄膜炎一例[J]. 中华皮肤科杂志, 2018, 51(5): 391-392.
[9]	张伟明韩丽娟曹俊周小勇段逸群. 成功救治甲氨蝶呤急性中毒反应一例[J]. 中华皮肤科杂志, 2018, 51(5): 385-387.
[10]	许昌春杜蕾蕾曾荣童建波刘宇甄段志敏陈旭徐浩翔龚春燕李岷. 白介素6在痤疮囊肿皮损中的表达及痤疮丙酸杆菌体外对THP-1细胞白介素6水平的影响[J]. 中华皮肤科杂志, 2018, 51(4): 265-268.
[11]	马蕾薛海波高梅兰舒春梅于娟张俊花. Notch1信号对银屑病患者外周血Th17细胞分化和功能的影响[J]. 中华皮肤科杂志, 2018, 51(3): 189-193.
[12]	徐鲁蒙王振华刘慧贤. 文身诱发银屑病一例[J]. 中华皮肤科杂志, 2018, 51(3): 233-233.
[13]	谢颖程苏云曾华松. 一例红皮病患儿白细胞介素36RN突变分析[J]. 中华皮肤科杂志, 2018, 51(12): 899-901.
[14]	郑瑀心闵敏朱子羽杨晓红曹毅郑敏. 甘草次酸对银屑病患者角质形成细胞增殖、凋亡及miR-21表达的影响[J]. 中华皮肤科杂志, 2018, 51(11): 802-805.
[15]	刘瑞风杨晓红梁见楠张开明. 银屑病患者皮损间充质干细胞环状RNA表达异常研究[J]. 中华皮肤科杂志, 2018, 51(10): 723-728.