中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (11): 753-757.

• 论著 • 上一篇    下一篇

黄芩素、阿维A诱导人皮肤鳞状细胞癌SCL-12细胞凋亡的研究

刘梅1,王珍2,肖汀3,王雅坤2,金光玉4,陈洪铎5,何春涤3   

  1. 1. 沈阳市中国医科大学附属第一医院皮肤科
    2. 沈阳 中国医科大学附属第一医院皮肤科
    3. 中国医科大学附属第一医院皮肤科
    4. 沈阳中国医科大学附属第一医院皮肤科
    5. 中国医科大学第一附属医院皮肤科
  • 收稿日期:2010-02-25 修回日期:2010-05-21 出版日期:2010-11-15 发布日期:2010-11-10
  • 通讯作者: 刘梅 E-mail:mayliu0115@gmail.com
  • 基金资助:

    辽宁省教育厅创新团队项目计划;辽宁省教育厅创新团队项目计划;教育部高等学校博士学科点专项科研基金;教育部创新团队基金

Apoptosis in cutaneous squamous cell carcinoma cell line SCL-12 induced by baicalein and acitretin

  • Received:2010-02-25 Revised:2010-05-21 Online:2010-11-15 Published:2010-11-10

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摘要：

目的 探讨黄芩素、阿维A对人皮肤鳞状细胞癌细胞株SCL-12细胞凋亡的影响。方法 不同浓度黄芩素、阿维A单独及联合作用于SCL-12细胞，噻唑蓝（MTT）法检测上述药物对SCL-12细胞的生长抑制作用；膜联蛋白Ⅴ-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双染法和ELISA法检测SCL-12细胞凋亡率；实时荧光定量RT-PCR法检测Fas mRNA的表达。结果 黄芩素、阿维A单独及联合应用均可抑制SCL-12细胞的生长，除3.125 μmol/L黄芩素与2.5 μmol/L阿维A联合用药组外，其余各联合用药组与单药作用组相比，抑制作用明显增强，且抑制作用随用药浓度的增加而增强。3.125 μmol/L黄芩素、5.0 μmol/L阿维A单独及联合应用48 h后均可诱导SCL-12细胞凋亡，早期凋亡率分别为9.39% ± 1.52%、20.86% ± 2.16%、36.85% ± 3.26%，与对照组（4.39% ± 0.64%）比较，差异均有统计学意义（P值均 < 0.05）；联合用药组对SCL-12细胞凋亡的诱导作用显著强于单药作用组，差异有统计学意义（F = 138.44，P < 0.05）。黄芩素、阿维A单独及联合应用48 h后均可上调SCL-12细胞Fas mRNA表达水平，联合用药组SCL-12细胞的Fas mRNA表达水平显著高于单药作用组。结论 黄芩素、阿维A单独及联合应用均能抑制SCL-12细胞生长并诱导其凋亡，两种药物的联合应用对SCL-12细胞具有协同效应，其发生机制可能与上调Fas凋亡调控基因的表达有关。

关键词: 黄芩素, 阿维A酸, 癌，鳞状细胞, 细胞凋亡

Abstract:

Objective To investigate the effects of baicalein and acitretin on the apoptosis in a human cutaneous squamous cell carcinoma cell line, SCL-12. Methods Cultured SCL-12 cells were treated with different concentrations of baicalein （3.125, 6.25, 12.5 μmol/L） and acitretin （2.5, 5.0, 10.0 μmol/L）, alone or in combination, for 48 hours. Subsequently, cell proliferation was detected by MTT assay, and cell apoptosis by ELISA as well as annexin V-FITC and propidium iodide double staining. Real-time quantitative RT-PCR was used to detect the expression of Fas mRNA in SCL-12 cells. Results The cell proliferation of SCL-12 cells was inhibited by baicalein and acitretin alone or in combination. The combination of baicalein and acitretin at the three tested concentrations, except for that of baicalein at 3.125 μmol/L and acitretin at 2.5 μmol/L, more strongly inhibited the proliferation of SCL-12 cells compared with baicalein or acitretin alone, and the inhibitory effect was in a dose-dependent manner. The early apoptosis rate was 9.39% ± 1.52%, 20.86% ± 2.16%, 36.85% ± 3.26% in SCL-12 cells treated with baicalein of 3.125 μmol/L, acitretin of 5.0 μmol/L alone and their combination, respectively, significantly higher than that in untreated cells （4.39% ± 0.64%, all P < 0.05）; the induction of apoptosis in SCL-12 cells by the combination of baicalein and acitretin was stronger than that by baicalein or acitretin alone （F = 138.44, P < 0.05）. Baicalein and acitretin alone or in combination significantly increased the mRNA expression of Fas in SCL-12 cells, and the effect of their combination was stronger than that of baicalein or acitretin alone. Conclusions Baicalein and acitretin could inhibit the growth of and induce the apoptosis in SCL-12 cells, and the effect is enhanced by the combination of baicalein and acitretin, which may be associated with the upregulation of Fas expression in SCL-12 cells.

Key words: Carcinoma, squamous cell, Apoptosis, Baicalein, Acitretin