中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (5): 321-323.

• 论著 • 上一篇    下一篇

CD1a和CD83分子在宫颈病变中的表达

赖亭吉1,骆丹2   

  1. 1. 南京医科大学第一附属医院
    2. 南京市南京医科大学附属第一医院皮肤科
  • 收稿日期:2008-11-10 修回日期:2009-03-05 出版日期:2009-05-15 发布日期:2009-05-13
  • 通讯作者: 赖亭吉

Expressions of CD1a and CD83 molecules in cervical lesions

  • Received:2008-11-10 Revised:2009-03-05 Online:2009-05-15 Published:2009-05-13

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摘要：

目的 探讨不同宫颈病变中CD1a和CD83分子的表达。方法 免疫组化法分别检测30例宫颈癌患者、30例宫颈尖锐湿疣患者和30例慢性宫颈炎上皮组织中CD1a和CD83分子的表达。结果 当宫颈病变向宫颈癌进展，瘤细胞侵袭性增加时，未成熟的CD1a+树突细胞密度随之降低（慢性宫颈炎3.45个细胞/HPF， 尖锐湿疣2.89个细胞/HPF，宫颈癌2.41个细胞/HPF）。相比而言，尖锐湿疣皮损与慢性宫颈炎相比，成熟的CD83+树突状细胞密度有显著提高（尖锐湿疣0.057个细胞/HPF，慢性宫颈炎0.012个细胞/HPF）。但在宫颈癌中成熟的CD83+树突状细胞密度也有一定的提高（宫颈癌0.034个细胞/HPF）。宫颈癌组织中，HPV16/18型阳性率为56.67%，HPV6/11型阳性率为3.30%；宫颈尖锐湿疣组织中，HPV6/11型阳性率为73.30%，HPV16/18型阳性率为6.67%；慢性宫颈炎组织中，HPV6/11型阳性率为3.30%，HPV16/18型阳性率为0。结论　宫颈癌组织中抗原提呈细胞尚不足以引起免疫反应防止病变的侵袭发展。

关键词: 宫颈病变;HPV;CD1a分子;CD83分子

Abstract:

Objective To investigate Expressions of CD1a and CD83 molecules in cervical lesions. Methods Immunohistochemical method was used to examine the expressions of CD1a and CD83 molecules in tissue samples from 30 patients with squamous cell carcinoma （SCC） of the cervix, 30 patients with cervical condyloma accuminatum （CA） and 30 patients with cervicitis. Results With the increase in the invasiveness of cervical lesions, there was a decline in the density of immature CD1a+ dendritic cells. The average number of immature CD1a+ dendritic cells per high power field （HPF） was 3.45 in cervicitis tissue, 2.89 in CA tissue, 2.41 in SCC tissue. On the contrast, a significant increase was observed in the density of mature CD83+ dendritic cells in CA tissue and SCC tissue compared with the cervicitis tissue （0.057 cells/HPF and 0.039 cells/HPF vs 0.019 cells/HPF, both P < 0.05）. The positivity rates of HPV 16/18 and HPV 6/11 were 56.67% and 3.30%, respectively, in cervical carcinoma tissue, 73.30% and 6.67%, respectively, in CA tissue, 3.30% and 0, respectively, in cervicitis tissue. Conclusions Compared with CA tissue, less mature dendritic cells were observed in cervical carcinoma tissue, demonstrating that the antigen presenting cells in carcinoma tissue are insufficient to mount an adequate immune response to prevent lesional invasion.