中华皮肤科杂志 ›› 2025, Vol. 58 ›› Issue (12): 1173-1179.doi: 10.35541/cjd.20240177

• 技术与方法 • 上一篇    下一篇

Droplet48法、微量肉汤稀释法和Sensititre YeastOne法体外检测念珠菌、隐球菌和曲霉菌药物敏感性的比较

潘开素1    郑冬燕1，2    吴维轩1    曹存巍1，3    刘京平1   

  1. 1广西医科大学第一附属医院皮肤科， 南宁  530022；2防城港市万清皮肤病与真菌病防控研究院，防城港  538000；3广西艾滋病防治研究重点实验室，南宁  530021
  • 收稿日期:2024-04-07 修回日期:2024-07-12 发布日期:2025-12-04
  • 通讯作者: 刘京平 E-mail:ljp003471@163.com
  • 基金资助:
    国家重点研发计划项目（2022YFC2504800）；广西创新研究团队（2020GXNSFGA238001）；广西科技基地和人才专项（桂科AD23026325）

Comparison of the Droplet48 method, microbroth dilution method, and Sensititre YeastOne method for antifungal susceptibility testing of Candida, Cryptococcus, and Aspergillus

Pan Kaisu1, Zheng Dongyan1,2, Wu Weixuan1, Cao Cunwei1,3, Liu Jingping1#br#   

  1. 1Department of Dermatology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530022, China; 2Fangchenggang Wanqing Institute of Mycosis Prevention and Control, Fangchenggang 538000,China; 3Guangxi Key Laboratory of AIDS Prevention and Treatment, Nanning 530021, China
  • Received:2024-04-07 Revised:2024-07-12 Published:2025-12-04
  • Contact: Liu Jingping E-mail:ljp003471@163.com
  • Supported by:
    National Key Research and Development Program of China （2022YFC2504800）; Guangxi Innovation Research Team （2020GXNSFGA238001）;Guangxi Science and Technology Base and Talent Special Program （AD23026325）

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摘要： 【摘要】 目的 比较分析Droplet48法与肉汤微量稀释法和Sensititre YeastOne法检测临床来源念珠菌、隐球菌和曲霉菌对9种抗真菌药物的敏感性结果的一致性。方法　临床收集念珠菌70株、曲霉菌40株及隐球菌24株，分别采用微量肉汤稀释法、Sensititre YeastOne法以及Droplet48法检测氟康唑、伏立康唑、伊曲康唑、泊沙康唑、两性霉素B、氟胞嘧啶、阿尼芬净、米卡芬净和卡泊芬净体外对上述菌株的最小抑菌浓度（MIC值），分别比较微量肉汤稀释法和Droplet48法以及Sensititre YeastOne法和Droplet48法MIC值的基本符合率，使用Spearman相关分析，通过计算Droplet48法和另两种参考方法MIC值的相关系数来评价不同方法间的一致性。结果　微量肉汤稀释法和Droplet48法检测念珠菌属、曲霉菌属和隐球菌属对9种抗真菌药物敏感性（MIC）的基本符合率分别为84.3% ~ 92.9%、85.0% ~ 92.5%和83.3% ~ 100%，Sensititre YeastOne法和Droplet48法检测的基本符合率分别为85.7% ~ 94.3%、90.0% ~ 95.0%和87.5% ~ 100%，Droplet48法与参考方法MIC值的相关系数为0.602 ~ 0.934，Spearman相关分析显示P值均＜0.05，与参考方法对比一致性好。结论　Droplet48法检测念珠菌属、曲霉菌属和隐球菌属的药物敏感性与微量肉汤稀释法和Sensititre YeastOne法一致性良好，是一种自动化程度更高且可靠的方法。

关键词: 微生物敏感性试验, Droplet48, 微量肉汤稀释法, Sensititre YeastOne

Abstract: 【Abstract】 Objective To compare and analyze the consistency of antifungal susceptibility of clinical isolates of Candida, Cryptococcus, and Aspergillus to 9 antifungal drugs between the Droplet48 method and the microbroth dilution method, as well as between the Droplet48 method and the Sensititre YeastOne method. Methods A total of 70 Candida strains, 40 Aspergillus strains, and 24 Cryptococcus strains isolated from clinical samples were tested for antifungal susceptibility. The minimum inhibitory concentrations （MICs） of fluconazole, voriconazole, itraconazole, posaconazole, amphotericin B, flucytosine, anidulafungin, micafungin, and caspofungin were determined using the microbroth dilution method, Sensititre YeastOne method, and Droplet48 method. The essential agreements of MIC values were evaluated between the microbroth dilution method and the Droplet48 method, as well as between the Sensititre YeastOne method and the Droplet48 method. Spearman's correlation analysis was carried out to evaluate the consistency between different methods by calculating the correlation coefficients of MIC values obtained by the Droplet48 method and the two reference methods. Results The essential agreements of MICs of the 9 antifungal drugs against Candida, Aspergillus, and Cryptococcus between the microbroth dilution method and the Droplet48 method were 84.3% - 92.9%, 85.0% - 92.5%, and 83.3% - 100%, respectively; the essential agreements between the Sensititre YeastOne method and the Droplet48 method for Candida, Aspergillus, and Cryptococcus were 85.7% - 94.3%, 90% - 95%, and 87.5% - 100%, respectively. The correlation coefficients of MIC values between the Droplet48 method and the reference methods ranged from 0.602 to 0.934. Spearman's correlation analysis showed that P values were all < 0.05, indicating good consistency of the Droplet48 method with the reference methods. Conclusion The Droplet48 method demonstrated good consistency with both the microbroth dilution method and the Sensititre YeastOne method in evaluating the antifungal susceptibility of Candida, Aspergillus, and Cryptococcus, proving to be a more automated and reliable approach.

Key words: Microbial sensitivity tests, Droplet48, Microbroth dilution method, Sensititre YeastOne

引用本文

潘开素 郑冬燕, 吴维轩 曹存巍, 刘京平. Droplet48法、微量肉汤稀释法和Sensititre YeastOne法体外检测念珠菌、隐球菌和曲霉菌药物敏感性的比较[J]. 中华皮肤科杂志, 2025,58(12):1173-1179. doi:10.35541/cjd.20240177

Pan Kaisu, Zheng Dongyan, Wu Weixuan, Cao Cunwei, Liu Jingping. Comparison of the Droplet48 method, microbroth dilution method, and Sensititre YeastOne method for antifungal susceptibility testing of Candida, Cryptococcus, and Aspergillus[J]. Chinese Journal of Dermatology, 2025, 58(12): 1173-1179.doi:10.35541/cjd.20240177