中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (1): 29-31.

• 论著 • 上一篇    下一篇

斑秃患者外周血CD4+CD25+Foxp3调节性T细胞及T淋巴细胞亚群的测定

陈小敏1,杨秀丽1,史维平1,等2   

  1. 1. 太原市山西省人民医院皮肤科
    2. 湖北省中山医院整形美容外科
  • 收稿日期:2007-01-15 修回日期:2007-05-08 发布日期:2008-01-31
  • 通讯作者: 陈小敏 E-mail:chenxmin510@sohu.com

  • Received:2007-01-15 Revised:2007-05-08 Published:2008-01-31

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摘要： 目的 研究斑秃患者外周血中CD4+CD25+调节性T细胞数量及CD4+、CD8+ T淋巴细胞亚群数量与斑秃疾病严重程度的关系。方法 对斑秃进行病情分组，以流式细胞仪检测17例重度、15例局限型斑秃患者和25例正常人对照者外周血中有功能活性的CD4+CD25+调节性T细胞（即CD4+CD25+ Foxp 3 T 细胞）在CD4+ T淋巴细胞中的比率，CD4+和CD8+占T淋巴细胞的比率。 结果 重度斑秃患者外周血中有功能活性的CD4+CD25+ Foxp 3 T细胞占CD4+ T细胞比率为0.54% ± 0.31%，显著低于正常人对照组（3.21% ± 0.76%）及局限型斑秃患者（2.71% ± 0.37%，P ＜ 0.001）；与正常人对照组比较，差异无统计学意义（P ＞ 0.05）。重度斑秃患者的CD4+占T淋巴细胞的比率为32.61% ± 3.48%，显著低于正常人对照组（43.0% ± 3.63%，P ＜ 0.001），而CD8+占T淋巴细胞的比率为40.96% ± 8.54%，显著高于正常人对照组（25.23% ± 2.14%，P ＜ 0.001）。局限型斑秃患者的此两项指标分别为41.25% ± 4.27%和26.6% ± 2.28%，与对照组差异无统计学意义（P ＞ 0.05）。重度斑秃患者的CD8+占T淋巴细胞的比率与CD4+CD25+ Foxp 3调节性 T 细胞占CD4+ T细胞的比率有负相关关系（r ＝ －0.94，P ＜ 0.001）。结论 重度斑秃可能与外周血中CD4+CD25+ T细胞数量的减少和功能活性的降低有关。

关键词: 斑秃, T淋巴细胞，调节性, CD4阳性T淋巴细胞, CD8阳性T淋巴细胞

Abstract: Objective To investigate the relationship between the severity of alopecia areata （AA）and frequencies of CD4+CD25+Foxp 3 T cells, CD4+ T cells, and CD8+ T cells in peripheral blood of AA patients. Methods Flow cytometry was employed to measure the percentages of CD25+Foxp 3 regulatory T cells and CD25+ T cells among CD4+ T cells, as well as the percentages of CD4+ T cells and CD8+ T cells among T cells from the peripheral blood of 17 severe AA patients, 15 local AA patients and 25 normal controls. Results The percentage of active CD4+CD25+Foxp 3 T cells was significantly lower in the peripheral blood of severe AA patients than that in the normal controls and local AA patients （0.54% ± 0.31% vs 3.21% ± 0.76%, 0.54% ± 0.31% vs 2.71% ± 0.37%, respectively, both P < 0.001）, while no significant difference was found between the latter two groups （P > 0.05）. In severe AA patients, the percentage of CD4+ T cells was significantly lower than that in normal controls （32.61% ± 3.48% vs 43.0% ± 3.63%, P < 0.001）, while the percentage of CD8+ T cells was significantly higher than that in normal controls （40.96% ± 8.54% vs 25.23% ± 2.14%, P < 0.001）. The percentages of CD4+ T cells and CD8+ T cells of local AA patients were 41.25% ± 4.27% and 26.60% ± 2.28%, respectively, without noticeable difference from those of normal controls （P > 0.05）. The percentage of CD8+ T cells of severe AA patients was negatively correlated to that of CD4+CD25+ Foxp 3 regulatory T cells （r = -0.94, P < 0.001）. Conclusion The severity of AA may be related to the decrease of percentage and functionality of CD4+CD25+ T cells in peripheral blood of these patients.