中华皮肤科杂志 ›› 2021, Vol. 54 ›› Issue (12): 1071-1076.doi: 10.35541/cjd.20200854

• 论著 • 上一篇    下一篇

基于16S rRNA基因测序的慢性自发性荨麻疹患者肠道菌群差异研究

闫慧敏1    杜笑2    孟令贺1    翁立硕1    曾三武1   

  1. 1天津市第一中心医院皮肤科  南开大学医学院  300192；2天津市中医药大学  301608
  • 收稿日期:2020-08-28 修回日期:2021-01-24 发布日期:2021-12-01
  • 通讯作者: 曾三武 E-mail:Sanwuzeng@sohu.com
  • 作者简介:2021年底前录用通知，结题需要

Analysis of differences in gut microbiota in patients with chronic spontaneous urticaria by 16S rRNA high-throughput sequencing

Yan Huimin1, Du Xiao2, Meng Linghe1, Weng Lishuo1, Zeng Sanwu1   

  1. 1Department of Dermatology, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin 300192, China; 2Tianjin University of Traditional Chinese Medicine, Tianjin 301608, China
  • Received:2020-08-28 Revised:2021-01-24 Published:2021-12-01
  • Contact: Zeng Sanwu E-mail:Sanwuzeng@sohu.com

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摘要： 【摘要】 目的 研究慢性自发性荨麻疹（CSU）患者和健康人的肠道菌群差异。方法 收集2019年1 - 12月在天津市第一中心医院皮肤科就诊的18例CSU患者（CSU组）及年龄、性别相匹配的18例健康对照（HC组）的粪便标本，提取总DNA，利用16S rRNA测序技术鉴定微生物种类，用生物信息学方法分析菌群差异。采用SPSS 23.0软件对试验数据进行统计学分析。结果 在α多样性方面，CSU组Observed OTU指数161.28 ± 35.47，Chao1指数161.31 ± 35.51，Shannon指数5.15 ± 0.47，Simpson指数0.94 ± 0.03，HC组分别为154.89 ± 54.46、154.92 ± 54.43、4.92 ± 0.88、0.91 ± 0.08，两组差异均无统计学意义（t = 0.417、0.417、0.952、1.116，均P > 0.05）。在β多样性方面，主成分分析显示，两组的第一主成分解释度为6.66%，第二主成分解释度为4.93%，两组菌群结构差异无统计学意义（P = 0.672）。CSU组、HC组肠道菌群Holdemania菌属相对丰度分别为0.04%、0.01%，两组间差异有统计学意义（P = 0.025）。结论 CSU患者与健康人肠道菌群存在一定差异。

关键词: 荨麻疹, 微生物群落, 胃肠道, RNA, 核糖体, 16S, 计算生物学, 慢性自发性荨麻疹

Abstract: 【Abstract】 Objective To investigate differences in gut microbiota between patients with chronic spontaneous urticaria （CSU） and healthy controls. Methods A total of 18 patients with CSU （CSU group）and 18 age- and gender-matched healthy controls（HC group） were enrolled from Department of Dermatology, Tianjin First Central Hospital between January 2019 and December 2019. Fecal samples were collected from these subjects, and total DNA was extracted. The 16S rRNA sequencing technology was used to identify microbial species in gut microbiota, and bioinformatics methods were applied to analyze differences in gut microbiota composition between the 2 groups. The SPSS 23.0 software was used for statistical analysis of the experimental data. Results In terms of α diversity, there was no significant difference in the Observed OTU index, Chao1 index, Shannon index or Simpson index between the CSU group （161.28 ± 35.47, 161.31 ± 35.51, 5.15 ± 0.47, 0.94 ± 0.03, respectively） and HC group （154.89 ± 54.46, 154.92 ± 54.43, 4.92 ± 0.88, 0.91 ± 0.08, respectively; t = 0.417, 0.417, 0.952, 1.116, respectively, all P > 0.05）. In terms of β diversity, principal component analysis showed that the first and second principal components explained 6.66% and 4.93% respectively, and there was no significant difference in the microbiota structure between the 2 groups （P = 0.672）. The relative abundance of the genus Holdemania in the gut microbiota significantly differed between the CSU group and HC group （0.04% vs. 0.01%, P = 0.025）. Conclusion The gut microbiota differs between the patients with CSU and healthy controls.

Key words: Urticaria, Microbial consortia, Gastrointestinal tract, RNA, ribosomal, 16S, Computational biology, Chronic spontaneous urticaria