中华皮肤科杂志 ›› 2019, Vol. 52 ›› Issue (2): 94-99.doi: 10.3760/cma.j.issn.0412-4030.2019.02.005

• 论著 • 上一篇    下一篇

白癜风患者血清nesfatin-1与白细胞介素26的表达水平及其意义

郭远    蔡羽恬    郭宁宁    李遇梅   

  1. 江苏大学附属医院皮肤科  江苏大学再生医学研究院,江苏镇江 212001
  • 收稿日期:2018-07-17 修回日期:2018-12-22 出版日期:2019-02-15 发布日期:2019-01-29
  • 通讯作者: 李遇梅 E-mail:yumeili@ujs.edu.cn
  • 基金资助:
    国家自然科学基金(81573053);江苏省“333工程”资助项目(BRA2015196);镇江市第七批科技计划项目(SS2015024)

Expression of nucleobindin 2-encoded satiety and fat-influencing protein-1 and interleukin-26 in the serum of patients with vitiligo and its significance

Guo Yuan, Cai Yutian, Guo Ningning, Li Yumei   

  1. Department of Dermatology, Affiliated Hospital of Jiangsu University, Institute of Regenerative Medicine of Jiangsu University, Zhenjiang 212001, China
  • Received:2018-07-17 Revised:2018-12-22 Online:2019-02-15 Published:2019-01-29
  • Contact: Li Yumei E-mail:yumeili@ujs.edu.cn
  • Supported by:
    National Natural Science Foundation of China (81573053); The “333” Project of Jiangsu Province (BRA2015196); Seventh Batch of Science and Technology Plan in Zhenjiang (SS2015024)

摘要: 【摘要】 目的 检测白癜风患者血清中摄食抑制因子(nesfatin?1)、白细胞介素 26(IL?26)的表达水平,研究其与焦虑状态、病情分期、皮疹分布类型的关系。方法 2017年3月至2018年9月选取江苏大学附属医院皮肤科门诊诊断为白癜风的患者123例,其中伴有焦虑状态者93例(白癜风焦虑组),无焦虑者30例(白癜风无焦虑组),30例健康对照者(健康对照组)。同时,在镇江市第五人民医院选取30例无白癜风、无其他自身免疫性疾病及高血压、糖尿病等基础疾病的焦虑患者(普通焦虑组)。采用酶联免疫吸附实验检测各组nesfatin?1及IL?26表达水平,采用焦虑状态特质问卷评估各组的焦虑状态。白癜风患者按疾病活动性评分分为稳定期、进展期和快速进展期,根据皮损分为局限型、节段型、泛发型、散发型、肢端型,分析各组、各型间nesfatin?1及IL?26水平。多组资料采用单因素方差分析,多个影响因素时采用多因素方差分析及分层分析,治疗前后比较采用配对t检验,相关性分析采用Pearson相关分析。结果 白癜风焦虑组、白癜风无焦虑组、普通焦虑组和对照组血清nesfatin?1含量差异有统计学意义(F = 10.78,P < 0.001),其中,白癜风焦虑组血清中nesfatin?1含量显著高于白癜风无焦虑组、普通焦虑组及对照组(P < 0.001)。4组间血清中IL?26含量差异无统计学意义(F = 1.34,P = 0.26)。Pearson相关分析显示,93例白癜风焦虑患者血清nesfatin?1水平与焦虑状态特质问卷评分呈正相关(r = 0.55,P < 0.001)。各型中快速进展期白癜风焦虑患者血清nesfatin?1水平均显著高于稳定期(P < 0.05),而不同分期白癜风焦虑患者 IL?26水平差异无统计学意义(P > 0.05)。各期中泛发型白癜风焦虑患者血清nesfatin?1水平均显著高于局限型(P < 0.001)。10例白癜风焦虑患者治疗3个月后nesfatin?1水平低于治疗前(配对t = 4.40,P = 0.02),但焦虑状态特质问卷评分无变化(P > 0.05)。结论 Nesfatin?1可能作为情绪影响因子参与了白癜风的发病,尤其作用于快速进展期和泛发型患者,而IL?26与白癜风发生发展关系不大。

关键词: 白癜风, 焦虑, 白细胞介素26, 摄食抑制因子1, 泛发型白癜风

Abstract: 【Abstract】 Objective To determine the expression of nucleobindin 2-encoded satiety and fat-influencing protein-1 (nesfatin-1) and interleukin (IL)-26 in the serum of patients with vitiligo, and to explore the relationship of nesfatin-1 and IL-26 with anxiety status, disease stage and distribution pattern of vitiligo lesions. Methods From March 2017 to September 2018, 123 outpatients with vitiligo, as well as 30 healthy controls (control group), were enrolled from Department of Dermatology, Affiliated Hospital of Jiangsu University. Of these patients, 93 rated as anxious (vitiligo with anxiety group), and 30 as non-anxious (vitiligo without anxiety group). Another 30 anxious patients with other autoimmune diseases and background diseases like hypertension and diabetes mellitus but not vitiligo (anxiety group) were enrolled from Zhenjiang Fifth People′s Hospital. Enzyme-linked immunosorbent assay (ELISA) was performed to detect levels of nesfatin-1 and IL-26 in the above groups, and state-trait anxiety inventory (STAI) was used to evaluate the anxiety status. According to the vitiligo disease activity (VIDA) score, the vitiligo patients with anxiety were divided into 3 subgroups: stable stage group, active stage group and rapid progressive stage group. According to the distribution pattern of skin lesions, the vitiligo patients with anxiety were divided into 5 subgroups: localized vitiligo group, segmental vitiligo group, generalized vitiligo group, sporadic vitiligo group and acral vitiligo group. The levels of nesfatin-1 and IL-26 were compared among different stage groups and type groups. Statistical analysis was carried out using one-way analysis of variance for comparison among several groups, multi-way analysis of variance and stratification analysis for multifactor data, paired t-test for comparison before and after treatment, and Pearson correlation analysis for analyzing correlations. Results The serum level of nesfatin-1 significantly differed among the vitiligo with anxiety group, vitiligo without anxiety group, anxiety group and control group (F = 10.78, P < 0.001), and was significantly higher in the vitiligo with anxiety group than in the other 3 groups (P < 0.001). No significant difference in the serum level of IL-26 was found among the 4 above groups (F = 1.34, P = 0.26). As pearson correlation analysis showed, the serum level of nesfatin-1 was positively correlated with the STAI score in the 93 vitiligo patients with anxiety (r = 0.55, P < 0.001). The serum level of nesfatin-1 was significantly higher in the rapid progressive stage group than in the stable stage group (P < 0.05), while there was no significant difference in the IL-26 level among different stage groups (P > 0.05). The generalized vitiligo patients with anxiety showed significantly increased serum level of nesfatin-1 compared with the localized vitiligo patients with anxiety (P < 0.001). After 3-month treatment, the nesfatin-1 level in the 10 vitiligo patients with anxiety significantly decreased compared with that before the treatment (paired t = 4.40, P = 0.02), but the STAI score did not change (P > 0.05). Conclusion Nesfatin-1 as an emotion-influencing factor may participate in the occurrence of vitiligo, especially affect patients with rapid progressive vitiligo or generalized vitiligo, while IL-26 may be irrelevant to vitiligo.

Key words: Vitiligo, Anxiety, Interleukin?26, Nesfatin?1, Generalized vitiligo